1xb2: Difference between revisions

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{{Seed}}
[[Image:1xb2.png|left|200px]]


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==Crystal Structure of Bos taurus mitochondrial Elongation Factor Tu/Ts Complex==
The line below this paragraph, containing "STRUCTURE_1xb2", creates the "Structure Box" on the page.
<StructureSection load='1xb2' size='340' side='right'caption='[[1xb2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1xb2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XB2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_1xb2|  PDB=1xb2  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xb2 OCA], [https://pdbe.org/1xb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xb2 RCSB], [https://www.ebi.ac.uk/pdbsum/1xb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xb2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EFTU_BOVIN EFTU_BOVIN] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xb/1xb2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xb2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structure of the bovine mitochondrial elongation factor (EF)-Tu.Ts complex (EF-Tumt.Tsmt) has been determined to 2.2-A resolution using the multi-wavelength anomalous dispersion experimental method. This complex provides the first insight into the structure of EF-Tsmt. EF-Tsmt is similar to Escherichia coli and Thermus thermophilus EF-Ts in the amino-terminal domain. However, the structure of EF-Tsmt deviates considerably in the core domain with a five-stranded beta-sheet forming a portion of subdomain N of the core. In E. coli EF-Ts, this region is composed of a three-stranded sheet. The coiled-coil domain of the E. coli EF-Ts is largely eroded in EF-Tsmt, in which it consists of a large loop packed against subdomain C of the core. The conformation of bovine EF-Tumt in complex with EF-Tsmt is distinct from its conformation in the EF-Tumt.GDP complex. When domain III of bovine EF-Tumt.GDP is superimposed on domain III of EF-Tumt in the EF-Tumt.Tsmt complex, helix B from domain I is also almost superimposed. However, the rest of domain I is rotated relative to this helix toward domain II, which itself is rotated toward domain I relative to domain III. Extensive contacts are observed between the amino-terminal domain of EF-Tsmt and domain I of EF-Tumt. Furthermore, the conserved TDFV sequence of EF-Tsmt also contacts domain I with the side chain of Asp139 contacting helix B of EF-Tumt and inserting the side chain of Phe140 between helices B and C. The structure of the EF-Tumt.Tsmt complex provides new insights into the nucleotide exchange mechanism and provides a framework for explaining much of the mutational data obtained for this complex.


===Crystal Structure of Bos taurus mitochondrial Elongation Factor Tu/Ts Complex===
Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex.,Jeppesen MG, Navratil T, Spremulli LL, Nyborg J J Biol Chem. 2005 Feb 11;280(6):5071-81. Epub 2004 Nov 22. PMID:15557323<ref>PMID:15557323</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1xb2" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_15557323}}, adds the Publication Abstract to the page
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 15557323 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_15557323}}
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</StructureSection>
==About this Structure==
1XB2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XB2 OCA].
 
==Reference==
Crystal structure of the bovine mitochondrial elongation factor Tu.Ts complex., Jeppesen MG, Navratil T, Spremulli LL, Nyborg J, J Biol Chem. 2005 Feb 11;280(6):5071-81. Epub 2004 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15557323 15557323]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Jeppesen, M G.]]
[[Category: Jeppesen MG]]
[[Category: Navratil, T.]]
[[Category: Navratil T]]
[[Category: Nyborg, J.]]
[[Category: Nyborg J]]
[[Category: Spremulli, L L.]]
[[Category: Spremulli LL]]
[[Category: Protein-protein complex]]
 
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Retrieved from "https://proteopedia.org/1xb2"