1qwf: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1qwf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwf" /> '''C-SRC SH3 DOMAIN COMPLEXED WITH LIGAND VSL12...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1qwf.jpg|left|200px]]<br /><applet load="1qwf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1qwf" />
'''C-SRC SH3 DOMAIN COMPLEXED WITH LIGAND VSL12'''<br />


==Overview==
==C-SRC SH3 DOMAIN COMPLEXED WITH LIGAND VSL12==
Two dodecapeptides belonging to distinct classes of Src homology 3 (SH3), ligands and selected from biased phage display libraries were used to, investigate interactions between a specificity pocket in the Src SH3, domain and ligant residues flanking the proline-rich core. The solution, structures of c-Src SH3 complexed with these peptides were solved by NMR., In addition to proline-rich, polyproline type II helix-forming core, the, class I and II ligands each possesses a flanking sequence that occupies a, large pocket between the RT and n-Src loops of the SH3 domain. Structural, and mutational analyses illustrate how the two classes of SH3 ligands, exploit a specificity pocket on the receptor differently to increase, binding affinity and specificity.
<StructureSection load='1qwf' size='340' side='right'caption='[[1qwf]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Avian_sarcoma_virus Avian sarcoma virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwf OCA], [https://pdbe.org/1qwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwf RCSB], [https://www.ebi.ac.uk/pdbsum/1qwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SRC_AVISR SRC_AVISR] This phosphoprotein, required for both the initiation and the maintenance of neoplastic transformation, is a protein kinase that catalyzes the phosphorylation of tyrosine residues in vitro.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwf ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Two dodecapeptides belonging to distinct classes of Src homology 3 (SH3) ligands and selected from biased phage display libraries were used to investigate interactions between a specificity pocket in the Src SH3 domain and ligant residues flanking the proline-rich core. The solution structures of c-Src SH3 complexed with these peptides were solved by NMR. In addition to proline-rich, polyproline type II helix-forming core, the class I and II ligands each possesses a flanking sequence that occupies a large pocket between the RT and n-Src loops of the SH3 domain. Structural and mutational analyses illustrate how the two classes of SH3 ligands exploit a specificity pocket on the receptor differently to increase binding affinity and specificity.


==About this Structure==
Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.,Feng S, Kasahara C, Rickles RJ, Schreiber SL Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12408-15. PMID:8618911<ref>PMID:8618911</ref>
1QWF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Avian_sarcoma_virus Avian sarcoma virus]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QWF OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands., Feng S, Kasahara C, Rickles RJ, Schreiber SL, Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12408-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8618911 8618911]
</div>
<div class="pdbe-citations 1qwf" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Tyrosine kinase 3D structures|Tyrosine kinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Avian sarcoma virus]]
[[Category: Avian sarcoma virus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Transferase]]
[[Category: Chiyoshi K]]
[[Category: Chiyoshi, K.]]
[[Category: Feng S]]
[[Category: Feng, S.]]
[[Category: Rickles RJ]]
[[Category: Rickles, R.J.]]
[[Category: Schreiber SL]]
[[Category: Schreiber, S.L.]]
[[Category: class i ligand complex]]
[[Category: src sh3 domain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:03:48 2007''
Retrieved from "https://proteopedia.org/1qwf"