2vp8: Difference between revisions

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{{Seed}}
[[Image:2vp8.jpg|left|200px]]


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==Structure of Mycobacterium tuberculosis Rv1207==
The line below this paragraph, containing "STRUCTURE_2vp8", creates the "Structure Box" on the page.
<StructureSection load='2vp8' size='340' side='right'caption='[[2vp8]], [[Resolution|resolution]] 2.64&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2vp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VP8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
{{STRUCTURE_2vp8|  PDB=2vp8  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vp8 OCA], [https://pdbe.org/2vp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vp8 RCSB], [https://www.ebi.ac.uk/pdbsum/2vp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vp8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHPS2_MYCTU DHPS2_MYCTU] Has very low affinity for the DHPS substrate 6-hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism.<ref>PMID:18680522</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vp8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vp8 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dihydropteroate synthase (DHPS) is involved in de novo biosynthesis of the essential cofactor folate by catalyzing the condensation of para-aminobenzoic acid (pABA) and 6-hydroxymethyl-7,8-dihydropterin-pyrophosphate (H2PtPP). Mycobacterium tuberculosis possesses a functional DHPS (MtDHPS, Rv3608c, folP1) and, based on sequence similarities, a putative ortholog (Rv1207, folP2). Here, we demonstrate that Rv1207 shows a low H2PtPP substrate affinity and lacks enzymatic DHPS activity. However, we found dapsone, a structural analog of pABA and clinically used DHPS inhibitor, to weakly bind both proteins. To gain insights into the lack of DHPS activity of Rv1207, its three-dimensional structure was determined at 2.64 A. The overall fold of both, MtDHPS (1EYE) and Rv1207, is highly conserved and conforms to a classical triosephosphate isomerase barrel arrangement. The predicted H2PtPP-binding pocket of Rv1207 is occupied by a histidine side chain, relative to a leucine residue in MtDHPS, consistent with the low affinity for this substrate and the lack of DHPS activity. We conclude that folP2 does not encode a DHPS and therefore cannot act as bypass for folP1. The metabolic function of Rv1207 remains to be defined.


===STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS RV1207===
Biochemical and structural characterization of the putative dihydropteroate synthase ortholog Rv1207 of Mycobacterium tuberculosis.,Gengenbacher M, Xu T, Niyomrattanakit P, Spraggon G, Dick T FEMS Microbiol Lett. 2008 Oct;287(1):128-35. Epub 2008 Aug 1. PMID:18680522<ref>PMID:18680522</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2vp8" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18680522}}, adds the Publication Abstract to the page
*[[Dihydropteroate synthase 3D structures|Dihydropteroate synthase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18680522 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18680522}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2VP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VP8 OCA].
[[Category: Mycobacterium tuberculosis H37Rv]]
 
[[Category: Dick T]]
==Reference==
[[Category: Gengenbacher M]]
Biochemical and structural characterization of the putative dihydropteroate synthase ortholog Rv1207 of Mycobacterium tuberculosis., Gengenbacher M, Xu T, Niyomrattanakit P, Spraggon G, Dick T, FEMS Microbiol Lett. 2008 Aug 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18680522 18680522]
[[Category: Niyomwattanakit P]]
 
[[Category: Spraggon G]]
Crystal structure of Mycobacterium tuberculosis 7,8-dihydropteroate synthase in complex with pterin monophosphate: new insight into the enzymatic mechanism and sulfa-drug action., Baca AM, Sirawaraporn R, Turley S, Sirawaraporn W, Hol WG, J Mol Biol. 2000 Oct 6;302(5):1193-212. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11007651 11007651]
[[Category: Xu T]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Single protein]]
[[Category: Transferase]]
[[Category: Dick, T.]]
[[Category: Gengenbacher, M.]]
[[Category: Niyomwattanakit, P.]]
[[Category: Spraggon, G.]]
[[Category: Xu, T.]]
[[Category: Antibiotic resistance]]
[[Category: Dihydropteroate synthase]]
[[Category: Folate biosynthesis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Rv1207]]
[[Category: Transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 13 14:10:47 2008''

Latest revision as of 15:27, 13 December 2023

Structure of Mycobacterium tuberculosis Rv1207

2vp8, resolution 2.64Å

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