1r2w: Difference between revisions

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New page: left|200px<br /><applet load="1r2w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r2w" /> '''Coordinates of L11 with 58nts of 23S rRNA fi...
 
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[[Image:1r2w.gif|left|200px]]<br /><applet load="1r2w" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1r2w" />
'''Coordinates of L11 with 58nts of 23S rRNA fitted into the cryo-EM map of the 70S ribosome'''<br />


==Overview==
==Coordinates of L11 with 58nts of 23S rRNA fitted into the cryo-EM map of the 70S ribosome==
Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the, ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we, present a cryo-electron microscopy (cryo-EM) study, at a resolution of, approximately 9 A, showing that during the incorporation of the aa-tRNA, into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA, allows the initial codon recognition and its accommodation into the, ribosomal A site. In addition, a conformational change observed in the, GTPase-associated center (GAC) of the ribosomal 50S subunit may provide, the mechanism by which the ribosome promotes a relative movement of the, aa-tRNA with respect to EF-Tu. This relative rearrangement seems to, facilitate codon recognition by the incoming aa-tRNA, and to provide the, codon-anticodon recognition-dependent signal for the GTPase activity of, EF-Tu. From these new findings we propose a mechanism that can explain the, sequence of events during the decoding of mRNA on the ribosome.
<SX load='1r2w' size='340' side='right' viewer='molstar' caption='[[1r2w]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1r2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R2W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r2w OCA], [https://pdbe.org/1r2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r2w RCSB], [https://www.ebi.ac.uk/pdbsum/1r2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r2w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r2w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r2w ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1R2W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R2W OCA].
*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
 
__TOC__
==Reference==
</SX>
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy., Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J, Nat Struct Biol. 2003 Nov;10(11):899-906. Epub 2003 Oct 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14566331 14566331]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Ehrenberg, M.]]
[[Category: Ehrenberg M]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Harvey, S.C.]]
[[Category: Harvey SC]]
[[Category: Li, W.]]
[[Category: Li W]]
[[Category: Nielsen, R.C.]]
[[Category: Nielsen RC]]
[[Category: Nissen, P.]]
[[Category: Nissen P]]
[[Category: Sengupta, J.]]
[[Category: Sengupta J]]
[[Category: Stagg, S.M.]]
[[Category: Stagg SM]]
[[Category: Valle, M.]]
[[Category: Valle M]]
[[Category: Zavialov, A.]]
[[Category: Zavialov A]]
[[Category: ribosomal protein]]
[[Category: rna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:13:47 2007''

Latest revision as of 08:20, 14 February 2024

Coordinates of L11 with 58nts of 23S rRNA fitted into the cryo-EM map of the 70S ribosome

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