2zqn: Difference between revisions

<table><tr><td colspan='2'>[[2zqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2d12 2d12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZQN FirstGlance]. <br>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zqn OCA], [https://pdbe.org/2zqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zqn RCSB], [https://www.ebi.ac.uk/pdbsum/2zqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zqn ProSAT]</span></td></tr>

R-type lectins are one of the most prominent types of lectin; they exist ubiquitously in nature and mainly bind to the galactose unit of sugar chains. The galactose-binding lectin EW29 from the earthworm Lumbricus terrestris belongs to the R-type lectin family as represented by the plant lectin ricin. It shows haemagglutination activity and is composed of a single peptide chain that includes two homologous domains: N-terminal and C-terminal domains. A truncated mutant of EW29 comprising the C-terminal domain (rC-half) has haemagglutination activity by itself. In order to clarify how rC-half recognizes ligands and shows haemagglutination activity, X-ray crystal structures of rC-half in complex with D-lactose and N-acetyl-D-galactosamine have been determined. The structure of rC-half is similar to that of the ricin B chain and consists of a beta-trefoil fold; the fold is further divided into three similar subdomains referred to as subdomains alpha, beta and gamma, which are gathered around the pseudo-threefold axis. The structures of sugar complexes demonstrated that subdomains alpha and gamma of rC-half bind terminal galactosyl and N-acetylgalactosaminyl glycans. The sugar-binding properties are common to both ligands in both subdomains and are quite similar to those of ricin B chain-lactose complexes. These results indicate that the C-terminal domain of EW29 uses these two galactose-binding sites for its function as a single-domain-type haemagglutinin.

Sugar-complex structures of the C-half domain of the galactose-binding lectin EW29 from the earthworm Lumbricus terrestris.,Suzuki R, Kuno A, Hasegawa T, Hirabayashi J, Kasai KI, Momma M, Fujimoto Z Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):49-57. Epub 2008, Dec 18. PMID:19153466<ref>PMID:19153466</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

<div class="pdbe-citations 2zqn" style="background-color:#fffaf0;"></div>

*[[Galactose-binding lectin|Galactose-binding lectin]]

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</StructureSection>

[[Category: Kuno A]]

[[Category: Momma M]]

[[Category: Suzuki R]]