3bsg: Difference between revisions

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{{Seed}}
[[Image:3bsg.jpg|left|200px]]


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==Barley alpha-amylase isozyme 1 (AMY1) H395A mutant==
The line below this paragraph, containing "STRUCTURE_3bsg", creates the "Structure Box" on the page.
<StructureSection load='3bsg' size='340' side='right'caption='[[3bsg]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3bsg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSG FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_3bsg|  PDB=3bsg  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsg OCA], [https://pdbe.org/3bsg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsg RCSB], [https://www.ebi.ac.uk/pdbsum/3bsg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMY1_HORVU AMY1_HORVU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bsg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bsg ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Certain starch hydrolases possess secondary carbohydrate binding sites outside of the active site, suggesting that multi-site substrate interactions are functionally significant. In barley alpha-amylase both Tyr380, situated on a remote non-catalytic domain, and Tyr105 in subsite -6 of the active site cleft are principal carbohydrate binding residues. The dual active site/secondary site mutants Y105A/Y380A and Y105A/Y380M show that each of Tyr380 and Tyr105 is important, albeit not essential for binding, degradation, and multiple attack on polysaccharides, while Tyr105 predominates in oligosaccharide hydrolysis. Additional delicate structure/function relationships of the secondary site are uncovered using Y380A/H395A, Y380A, and H395A AMY1 mutants.


===Barley alpha-amylase isozyme 1 (AMY1) H395A mutant===
Multi-site substrate binding and interplay in barley alpha-amylase 1.,Nielsen MM, Seo ES, Bozonnet S, Aghajari N, Robert X, Haser R, Svensson B FEBS Lett. 2008 Jul 23;582(17):2567-71. Epub 2008 Jun 25. PMID:18588886<ref>PMID:18588886</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3bsg" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18588886}}, adds the Publication Abstract to the page
*[[Amylase 3D structures|Amylase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18588886 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18588886}}
__TOC__
 
</StructureSection>
==About this Structure==
3BSG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSG OCA].
 
==Reference==
Multi-site substrate binding and interplay in barley alpha-amylase 1., Nielsen MM, Seo ES, Bozonnet S, Aghajari N, Robert X, Haser R, Svensson B, FEBS Lett. 2008 Jul 23;582(17):2567-71. Epub 2008 Jun 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18588886 18588886]
[[Category: Alpha-amylase]]
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Aghajari, N.]]
[[Category: Aghajari N]]
[[Category: Haser, R.]]
[[Category: Haser R]]
[[Category: Robert, X.]]
[[Category: Robert X]]
[[Category: Amy1]]
[[Category: Barley alpha-amylase]]
[[Category: Calcium]]
[[Category: Carbohydrate metabolism]]
[[Category: Germination]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Metal-binding]]
[[Category: Mutant]]
[[Category: Secreted]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 27 11:20:02 2008''

Latest revision as of 14:50, 1 November 2023

Barley alpha-amylase isozyme 1 (AMY1) H395A mutant

3bsg, resolution 1.95Å

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