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New page: left|200px<br /><applet load="1rca" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rca, resolution 1.9Å" /> '''STRUCTURE OF THE CRYS...
 
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[[Image:1rca.jpg|left|200px]]<br /><applet load="1rca" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1rca, resolution 1.9&Aring;" />
'''STRUCTURE OF THE CRYSTALLINE COMPLEX OF DEOXYCYTIDYLYL-3',5'-GUANOSINE (3',5'-DCPDG) CO-CRYSTALISED WITH RIBONUCLEASE AT 1.9 ANGSTROMS RESOLUTION. RETROBINDING IN PANCREATIC RNASEA IS INDEPENDENT OF MODE OF INHIBITOR INTROMISSION'''<br />


==Overview==
==STRUCTURE OF THE CRYSTALLINE COMPLEX OF DEOXYCYTIDYLYL-3',5'-GUANOSINE (3',5'-DCPDG) CO-CRYSTALISED WITH RIBONUCLEASE AT 1.9 ANGSTROMS RESOLUTION. RETROBINDING IN PANCREATIC RNASEA IS INDEPENDENT OF MODE OF INHIBITOR INTROMISSION==
The X-ray structure of bovine ribonuclease A cocrystallized with the, dinucleotide deoxycytidylyl-3',5'-guanosine has been determined at 1.9 A, resolution and refined by restrained least squares to R = 0.218 for 7807, reflections. The structure established that the recently observed, retrobound mode of attachment of substrate analogues, cytidylyl-2',5'-guanosine and deoxycytidylyl-3',5'-guanosine found in, soaked RNase A crystals is also present in the cocrystallized complex., Retrobinding is thus unlikely to be the result of restrictions imposed by, the crystalline environment as the ligands soak into the lattice but, rather a phenomenon specific to small nucleotides containing guanine.
<StructureSection load='1rca' size='340' side='right'caption='[[1rca]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1rca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CGP:2-DEOXYCYTIDINE-2-DEOXYGUANOSINE-3,5-MONOPHOSPHATE'>CGP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rca OCA], [https://pdbe.org/1rca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rca RCSB], [https://www.ebi.ac.uk/pdbsum/1rca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rca ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rca_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rca ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray structure of bovine ribonuclease A cocrystallized with the dinucleotide deoxycytidylyl-3',5'-guanosine has been determined at 1.9 A resolution and refined by restrained least squares to R = 0.218 for 7807 reflections. The structure established that the recently observed retrobound mode of attachment of substrate analogues cytidylyl-2',5'-guanosine and deoxycytidylyl-3',5'-guanosine found in soaked RNase A crystals is also present in the cocrystallized complex. Retrobinding is thus unlikely to be the result of restrictions imposed by the crystalline environment as the ligands soak into the lattice but rather a phenomenon specific to small nucleotides containing guanine.


==About this Structure==
Structure of the crystalline complex of deoxycytidylyl-3',5'-guanosine (3',5'-dCpdG) cocrystallized with ribonuclease at 1.9 A resolution.,Listgarten JN, Maes D, Wyns L, Aguilar CF, Palmer RA Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):767-71. PMID:15299807<ref>PMID:15299807</ref>
1RCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with PO4 and CGP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RCA OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of the crystalline complex of deoxycytidylyl-3',5'-guanosine (3',5'-dCpdG) cocrystallized with ribonuclease at 1.9 A resolution., Listgarten JN, Maes D, Wyns L, Aguilar CF, Palmer RA, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):767-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299807 15299807]
</div>
<div class="pdbe-citations 1rca" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Pancreatic ribonuclease]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Lisgarten JN]]
[[Category: Lisgarten, J.N.]]
[[Category: Palmer RA]]
[[Category: Palmer, R.A.]]
[[Category: CGP]]
[[Category: PO4]]
[[Category: hydrolase (endoribonuclease)]]
 
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Retrieved from "https://proteopedia.org/1rca"