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New page: left|200px<br /><applet load="1rit" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rit, resolution 2.85Å" /> '''Crystal structure of...
 
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[[Image:1rit.gif|left|200px]]<br /><applet load="1rit" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1rit, resolution 2.85&Aring;" />
'''Crystal structure of Peanut lectin in complex with meso-tetrasulphonatophenylporphyrin and lactose'''<br />


==Overview==
==Crystal structure of Peanut lectin in complex with meso-tetrasulphonatophenylporphyrin and lactose==
The extraordinary recognition specificity of lectins for carbohydrate, ligands appears to be violated as they also bind to porphyrins and other, noncarbohydrate ligands. In this study, crystal structures of, meso-tetrasulfonatophenylporphyrin (H(2)TPPS) bound to peanut agglutinin, (PNA) in the presence and absence of lactose were determined. The binding, of H(2)TPPS with PNA involved 11 molecules of H(2)TPPS in different, supramolecular stacking arrangements associated with a tetramer of PNA in, the crystals of the PNA-H(2)TPPS binary complex as well as the, PNA-H(2)TPPS-lactose ternary complex. The ternary complex involved lactose, binding only to two subunits of the PNA tetramer, which did not have, porphyrin interacting in the vicinity of the carbohydrate-binding site., Comparison of the two structures highlighted the plasticity of the, carbohydrate-binding site expressed in terms of the conformational change, in lactose binding. The unusual quaternary structure of PNA, which results, in exposed protein-protein interaction sites, might be responsible for the, porphyrin binding. The association of porphyrin in diverse oligomeric, stacking arrangements observed in the PNA-H(2)TPPS complex suggested the, possibility of protein-porphyrin aggregation under abnormal physiological, conditions. The structures described here provide a possible native, conformation of the carbohydrate-binding site of PNA in the absence of the, ligand, highlight mapping of the unsaturated binding surfaces of PNA using, porphyrin interactions, indicate new leads toward possible application of, this lectin in photodynamic therapy, and exhibit diverse modes of, porphyrin-lectin interactions with implications to porphyria, a disease, that results from abnormal accumulation of porphyrins.
<StructureSection load='1rit' size='340' side='right'caption='[[1rit]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1rit]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RIT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=SFP:5,10,15,20-TETRAKIS(4-SULPFONATOPHENYL)-21H,23H-PORPHINE'>SFP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rit OCA], [https://pdbe.org/1rit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rit RCSB], [https://www.ebi.ac.uk/pdbsum/1rit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rit ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECG_ARAHY LECG_ARAHY] D-galactose specific lectin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1rit_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rit ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1RIT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea] with LAT, CA, MN and SFP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RIT OCA].
*[[Galactose-binding lectin|Galactose-binding lectin]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of the PNA-porphyrin complex in the presence and absence of lactose: mapping the conformational changes on lactose binding, interacting surfaces, and supramolecular aggregations., Goel M, Damai RS, Sethi DK, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Biochemistry. 2005 Apr 19;44(15):5588-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15823017 15823017]
[[Category: Arachis hypogaea]]
[[Category: Arachis hypogaea]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Goel, M.]]
[[Category: Goel M]]
[[Category: Kaur, K.J.]]
[[Category: Kaur KJ]]
[[Category: Maiya, B.G.]]
[[Category: Maiya BG]]
[[Category: Salunke, D.M.]]
[[Category: Salunke DM]]
[[Category: Swamy, M.J.]]
[[Category: Swamy MJ]]
[[Category: CA]]
[[Category: LAT]]
[[Category: MN]]
[[Category: SFP]]
[[Category: sugar binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:38:03 2007''
Retrieved from "https://proteopedia.org/1rit"