3ebk: Difference between revisions
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==Crystal structure of major allergens, Bla g 4 from cockroaches== | |||
<StructureSection load='3ebk' size='340' side='right'caption='[[3ebk]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3ebk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Blattella_germanica Blattella germanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EBK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ebk OCA], [https://pdbe.org/3ebk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ebk RCSB], [https://www.ebi.ac.uk/pdbsum/3ebk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ebk ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/BLG4_BLAGE BLG4_BLAGE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Inhalant allergens from cockroaches are an important cause of asthma to millions of individuals worldwide. Here we report for the first time the structures of two major cockroach allergens, Bla g 4 and Per a 4, that adopt a typical lipocalin fold but with distinct structural features as compared with other known lipocalin allergens. Both Bla g 4 and Per a 4 contain two long-range disulfide bonds linking the N and C termini to a beta-barrel. The C-terminal helix of Bla g 4 is bent and greatly extended toward the N terminus. Bla g 4 is found to be a monomer, whereas Per a 4 exists as a dimer in solution with a novel dimeric interface involving residues from loops at the top and bottom of the beta-barrel. Putative ligand binding sites of both allergens are determined by docking of the juvenile hormone III inside the beta-barrel and found to interact with the ligand using non-conserved residues. Bla g 4 and Per a 4 are found to be cross-reactive in sera IgE binding, at least in the Singaporean Chinese population tested. A major IgE binding epitope unique to Per a 4 is found on the loops at the bottom of the beta-barrel that may aid the development of hypoallergens for immunotherapy. | |||
Structures of Two Major Allergens, Bla g 4 and Per a 4, from Cockroaches and Their IgE Binding Epitopes.,Tan YW, Chan SL, Ong TC, Yit le Y, Tiong YS, Chew FT, Sivaraman J, Mok YK J Biol Chem. 2009 Jan 30;284(5):3148-57. Epub 2008 Dec 4. PMID:19056737<ref>PMID:19056737</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3ebk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Blattella germanica]] | |||
[[Category: Large Structures]] | |||
[[Category: Chan SL]] | |||
[[Category: Chew FT]] | |||
[[Category: Mok YK]] | |||
[[Category: Sivaraman J]] | |||
[[Category: Tan YW]] | |||
Latest revision as of 11:57, 22 November 2023
Crystal structure of major allergens, Bla g 4 from cockroaches
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