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New page: left|200px<br /><applet load="1rnc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rnc, resolution 1.5Å" /> '''NEWLY OBSERVED BINDIN...
 
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[[Image:1rnc.gif|left|200px]]<br /><applet load="1rnc" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1rnc, resolution 1.5&Aring;" />
'''NEWLY OBSERVED BINDING MODE IN PANCREATIC RIBONUCLEASE'''<br />


==Overview==
==NEWLY OBSERVED BINDING MODE IN PANCREATIC RIBONUCLEASE==
Dinucleotides containing guanine, when soaked into crystals of bovine, pancreatic ribonuclease, have been found to bind in an unexpected manner, quite unlike interpretations of earlier X-ray diffraction studies. This, finding has prompted a reexamination of three mononucleotide-RNase, complexes from this laboratory resulting in a re-interpretation of the, complex that involved a guanine mononucleotide.
<StructureSection load='1rnc' size='340' side='right'caption='[[1rnc]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1rnc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RNC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rnc OCA], [https://pdbe.org/1rnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rnc RCSB], [https://www.ebi.ac.uk/pdbsum/1rnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rnc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/1rnc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rnc ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dinucleotides containing guanine, when soaked into crystals of bovine pancreatic ribonuclease, have been found to bind in an unexpected manner, quite unlike interpretations of earlier X-ray diffraction studies. This finding has prompted a reexamination of three mononucleotide-RNase complexes from this laboratory resulting in a re-interpretation of the complex that involved a guanine mononucleotide.


==About this Structure==
Newly observed binding mode in pancreatic ribonuclease.,Aguilar CF, Thomas PJ, Mills A, Moss DS, Palmer RA J Mol Biol. 1992 Mar 5;224(1):265-7. PMID:1548704<ref>PMID:1548704</ref>
1RNC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 and 5GP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RNC OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Newly observed binding mode in pancreatic ribonuclease., Aguilar CF, Thomas PJ, Mills A, Moss DS, Palmer RA, J Mol Biol. 1992 Mar 5;224(1):265-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1548704 1548704]
</div>
<div class="pdbe-citations 1rnc" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Pancreatic ribonuclease]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Aguilar CF]]
[[Category: Aguilar, C.F.]]
[[Category: Mills A]]
[[Category: Mills, A.]]
[[Category: Moss DS]]
[[Category: Moss, D.S.]]
[[Category: Palmer RA]]
[[Category: Palmer, R.A.]]
[[Category: Thomas PJ]]
[[Category: Thomas, P.J.]]
[[Category: 5GP]]
[[Category: SO4]]
[[Category: hydrolase(endoribonuclease)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:43:42 2007''

Latest revision as of 07:25, 9 October 2024

NEWLY OBSERVED BINDING MODE IN PANCREATIC RIBONUCLEASE

1rnc, resolution 1.50Å

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