2vax: Difference between revisions

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{{Seed}}
[[Image:2vax.jpg|left|200px]]


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==Crystal structure of deacetylcephalosporin C acetyltransferase (Cephalosporin C-soak)==
The line below this paragraph, containing "STRUCTURE_2vax", creates the "Structure Box" on the page.
<StructureSection load='2vax' size='340' side='right'caption='[[2vax]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2vax]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Acremonium_chrysogenum Acremonium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VAX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CSC:4-(3-ACETOXYMETHYL-2-CARBOXY-8-OXO-5-THIA-1-AZA-BICYCLO[4.2.0]OCT-2-EN-7-YLCARBAMOYL)-1-CARBOXY-BUTYL-AMMONIUM'>CSC</scene>, <scene name='pdbligand=OAS:O-ACETYLSERINE'>OAS</scene></td></tr>
{{STRUCTURE_2vax|  PDB=2vax  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vax OCA], [https://pdbe.org/2vax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vax RCSB], [https://www.ebi.ac.uk/pdbsum/2vax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vax ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CEFG_HAPCH CEFG_HAPCH] Catalyzes the conversion of deacetylcephalosporin C to cephalosporin C.<ref>PMID:1569032</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2vax_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vax ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Deacetylcephalosporin C acetyltransferase (DAC-AT) catalyses the last step in the biosynthesis of cephalosporin C, a broad-spectrum beta-lactam antibiotic of large clinical importance. The acetyl transfer step has been suggested to be limiting for cephalosporin C biosynthesis, but has so far escaped detailed structural analysis. We present here the crystal structures of DAC-AT in complexes with reaction intermediates, providing crystallographic snapshots of the reaction mechanism. The enzyme is found to belong to the alpha/beta hydrolase class of acetyltransferases, and the structures support previous observations of a double displacement mechanism for the acetyl transfer reaction in other members of this class of enzymes. The structures of DAC-AT reported here provide evidence of a stable acyl-enzyme complex, thus underpinning a mechanism involving acetylation of a catalytic serine residue by acetyl coenzyme A, followed by transfer of the acetyl group to deacetylcephalosporin C through a suggested tetrahedral transition state.


===CRYSTAL STRUCTURE OF DEACETYLCEPHALOSPORIN C ACETYLTRANSFERASE (CEPHALOSPORIN C-SOAK)===
The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates.,Lejon S, Ellis J, Valegard K J Mol Biol. 2008 Mar 28;377(3):935-44. Epub 2008 Jan 30. PMID:18279889<ref>PMID:18279889</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
2VAX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acremonium_chrysogenum Acremonium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAX OCA].
<div class="pdbe-citations 2vax" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acremonium chrysogenum]]
[[Category: Acremonium chrysogenum]]
[[Category: Deacetylcephalosporin-C acetyltransferase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Ellis J]]
[[Category: Ellis, J.]]
[[Category: Lejon S]]
[[Category: Lejon, S.]]
[[Category: Valegard K]]
[[Category: Valegard, K.]]
[[Category: A/b-hydrolase fold]]
[[Category: Acetyl transferase]]
[[Category: Acyltransferase]]
[[Category: Antibiotic biosynthesis]]
[[Category: Cephalosporin biosynthesis]]
[[Category: Deacetylcephalosporin c]]
[[Category: Transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 24 10:33:56 2008''

Latest revision as of 09:31, 6 November 2024

Crystal structure of deacetylcephalosporin C acetyltransferase (Cephalosporin C-soak)

2vax, resolution 2.60Å

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