1s2e: Difference between revisions

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New page: left|200px<br /><applet load="1s2e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s2e, resolution 2.30Å" /> '''BACTERIOPHAGE T4 GEN...
 
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[[Image:1s2e.jpg|left|200px]]<br /><applet load="1s2e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1s2e, resolution 2.30&Aring;" />
'''BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRACTION AND THE LONG TAIL FIBERS CONNECTOR, ALTERNATIVE FIT OF THE FIRST 19 RESIDUES'''<br />


==Overview==
==BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRACTION AND THE LONG TAIL FIBERS CONNECTOR, ALTERNATIVE FIT OF THE FIRST 19 RESIDUES==
BACKGROUND: The T4 bacteriophage consists of a head, filled with, double-stranded DNA, and a complex contractile tail required for the, ejection of the viral genome into the Escherichia coli host. The tail has, a baseplate to which are attached six long and six short tail fibers., These fibers are the sensing devices for recognizing the host. When, activated by attachment to cell receptors, the fibers cause a, conformational transition in the baseplate and subsequently in the tail, sheath, which initiates DNA ejection. The baseplate is a multisubunit, complex of proteins encoded by 15 genes. Gene product 9 (gp9) is the, protein that connects the long tail fibers to the baseplate and triggers, the tail contraction after virus attachment to a host cell. RESULTS: The, crystal structure of recombinant gp9, determined to 2.3 A resolution, shows that the protein of 288 amino acid residues assembles as a, homotrimer. The monomer consists of three domains: the N-terminal domain, generates a triple coiled coil; the middle domain is a mixed, seven-stranded beta sandwich with a topology not previously observed; and, the C-terminal domain is an eight-stranded, antiparallel beta sandwich, having some resemblance to 'jelly-roll' viral capsid protein structures., CONCLUSIONS: The biologically active form of gp9 is a trimer. The protein, contains flexible interdomain hinges, which are presumably required to, facilitate signal transmission between the long tail fibers and the, baseplate. Structural and genetic analyses show that the C-terminal domain, is bound to the baseplate, and the N-terminal coiled-coil domain is, associated with the long tail fibers.
<StructureSection load='1s2e' size='340' side='right'caption='[[1s2e]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1s2e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S2E FirstGlance]. <br>
1S2E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with EPE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S2E OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2e OCA], [https://pdbe.org/1s2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s2e RCSB], [https://www.ebi.ac.uk/pdbsum/1s2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s2e ProSAT]</span></td></tr>
The structure of bacteriophage T4 gene product 9: the trigger for tail contraction., Kostyuchenko VA, Navruzbekov GA, Kurochkina LP, Strelkov SV, Mesyanzhinov VV, Rossmann MG, Structure. 1999 Oct 15;7(10):1213-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10545330 10545330]
</table>
[[Category: Bacteriophage t4]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/BP09_BPT4 BP09_BPT4] Baseplate protein that connects the long tail fibers to the baseplate and probably triggers the tail contraction after virus attachment to a host cell (PubMed:10545330). Involved in the tail assembly (PubMed:21129200).<ref>PMID:10545330</ref> <ref>PMID:21129200</ref>
[[Category: Kostyuchenko, V.A.]]
== Evolutionary Conservation ==
[[Category: Kurochkina, L.P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Mesyanzhinov, V.V.]]
Check<jmol>
[[Category: Navruzbekov, G.A.]]
  <jmolCheckbox>
[[Category: Rossmann, M.G.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s2/1s2e_consurf.spt"</scriptWhenChecked>
[[Category: Strelkov, S.V.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: EPE]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: bacteriophage t4]]
  </jmolCheckbox>
[[Category: baseplate]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s2e ConSurf].
[[Category: gene product 9]]
<div style="clear:both"></div>
[[Category: oligomerization]]
== References ==
[[Category: virus/viral protein]]
<references/>
[[Category: x-ray crystallography]]
__TOC__
 
</StructureSection>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:03:05 2007''
[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Kostyuchenko VA]]
[[Category: Kurochkina LP]]
[[Category: Mesyanzhinov VV]]
[[Category: Navruzbekov GA]]
[[Category: Rossmann MG]]
[[Category: Strelkov SV]]

Latest revision as of 08:27, 14 February 2024

BACTERIOPHAGE T4 GENE PRODUCT 9 (GP9), THE TRIGGER OF TAIL CONTRACTION AND THE LONG TAIL FIBERS CONNECTOR, ALTERNATIVE FIT OF THE FIRST 19 RESIDUES

1s2e, resolution 2.30Å

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