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New page: left|200px<br /><applet load="1sa3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sa3, resolution 1.95Å" /> '''An asymmetric comple...
 
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[[Image:1sa3.gif|left|200px]]<br /><applet load="1sa3" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sa3, resolution 1.95&Aring;" />
'''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site'''<br />


==Overview==
==An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site==
Most well-known restriction endonucleases recognize palindromic DNA, sequences and are classified as Type IIP. Due to the recognition and, cleavage symmetry, Type IIP enzymes are usually found to act as homodimers, in forming 2-fold symmetric enzyme-DNA complexes. Here we report an, asymmetric complex of the Type IIP restriction enzyme MspI in complex with, its cognate recognition sequence. Unlike any other Type IIP enzyme, reported to date, an MspI monomer and not a dimer binds to a palindromic, DNA sequence. The enzyme makes specific contacts with all 4 base pairs in, the recognition sequence, by six direct and five water-mediated hydrogen, bonds and numerous van der Waal contacts. This MspI-DNA structure, represents the first example of asymmetric recognition of a palindromic, DNA sequence by two different structural motifs in one polypeptide. A few, possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
<StructureSection load='1sa3' size='340' side='right'caption='[[1sa3]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sa3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SA3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sa3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sa3 OCA], [https://pdbe.org/1sa3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sa3 RCSB], [https://www.ebi.ac.uk/pdbsum/1sa3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sa3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2M1_MORSP T2M1_MORSP] Recognizes the double-stranded sequence CCGG and cleaves after C-1.


==About this Structure==
==See Also==
1SA3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341737 15341737]
[[Category: Large Structures]]
[[Category: Moraxella sp.]]
[[Category: Moraxella sp]]
[[Category: Single protein]]
[[Category: Guo HC]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Kucera RB]]
[[Category: Guo, H.C.]]
[[Category: Roberts RJ]]
[[Category: Kucera, R.B.]]
[[Category: Xu QS]]
[[Category: Roberts, R.J.]]
[[Category: Xu, Q.S.]]
[[Category: NA]]
[[Category: protein-dna complex]]
 
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