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New page: left|200px<br /><applet load="1sff" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sff, resolution 1.9Å" /> '''Structure of gamma-am...
 
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[[Image:1sff.gif|left|200px]]<br /><applet load="1sff" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sff, resolution 1.9&Aring;" />
'''Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate'''<br />


==Overview==
==Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate==
The X-ray crystal structures of Escherichia coli gamma-aminobutyrate, aminotransferase unbound and bound to the inhibitor aminooxyacetate are, reported. The enzyme crystallizes from ammonium sulfate solutions in the, P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction, data were collected to 2.4 A resolution for the unliganded enzyme and 1.9, A resolution for the aminooxyacetate complex. The overall structure of the, enzyme is similar to those of other aminotransferase subgroup II enzymes., The ability of gamma-aminobutyrate aminotransferase to act on primary, amine substrates (gamma-aminobutyrate) in the first half-reaction and, alpha-amino acids in the second is proposed to be enabled by the presence, of Glu211, whose side chain carboxylate alternates between interactions, with Arg398 in the primary amine half-reaction and an alternative binding, site in the alpha-amino acid half-reaction, in which Arg398 binds the, substrate alpha-carboxylate. The specificity for a carboxylate group on, the substrate side chain is due primarily to the presence of Arg141, but, also requires substantial local main chain rearrangements relative to the, structurally homologous enzyme dialkylglycine decarboxylase, which is, specific for small alkyl side chains. No iron-sulfur cluster is found in, the bacterial enzyme as was found in the pig enzyme [Storici, P., De, Biase, D., Bossa, F., Bruno, S., Mozzarelli, A., Peneff, C., Silverman, R., B., and Schirmer, T. (2004) J. Biol. Chem. 279, 363-73.]. The binding of, aminooxyacetate causes remarkably small changes in the active site, structure, and no large domain movements are observed. Active site, structure comparisons with pig gamma-aminobutyrate aminotransferase and, dialkylglycine decarboxylase are discussed.
<StructureSection load='1sff' size='340' side='right'caption='[[1sff]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sff]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IK2:4-DEOXY-4-ACETYLYAMINO-PYRIDOXAL-5-PHOSPHATE'>IK2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sff OCA], [https://pdbe.org/1sff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sff RCSB], [https://www.ebi.ac.uk/pdbsum/1sff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sff ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GABT_ECOLI GABT_ECOLI] Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA).<ref>PMID:20639325</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sff_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sff ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1SFF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, EDO and IK2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-aminobutyrate_transaminase 4-aminobutyrate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.19 2.6.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SFF OCA].
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase., Liu W, Peterson PE, Carter RJ, Zhou X, Langston JA, Fisher AJ, Toney MD, Biochemistry. 2004 Aug 31;43(34):10896-905. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15323550 15323550]
__TOC__
[[Category: 4-aminobutyrate transaminase]]
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Carter, R.J.]]
[[Category: Carter RJ]]
[[Category: Fisher, A.J.]]
[[Category: Fisher AJ]]
[[Category: Langston, J.A.]]
[[Category: Langston JA]]
[[Category: Liu, W.]]
[[Category: Liu W]]
[[Category: Peterson, P.E.]]
[[Category: Peterson PE]]
[[Category: Toney, M.D.]]
[[Category: Toney MD]]
[[Category: Zhou, X.]]
[[Category: Zhou X]]
[[Category: EDO]]
[[Category: IK2]]
[[Category: SO4]]
[[Category: aminotransferase]]
[[Category: enzyme complexes]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:20:45 2007''

Latest revision as of 08:30, 14 February 2024

Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate

1sff, resolution 1.90Å

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