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New page: left|200px<br /><applet load="1sg7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sg7" /> '''NMR solution structure of the putative catio...
 
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[[Image:1sg7.gif|left|200px]]<br /><applet load="1sg7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sg7" />
'''NMR solution structure of the putative cation transport regulator ChaB'''<br />


==Overview==
==NMR solution structure of the putative cation transport regulator ChaB==
BACKGROUND: ChaB is a putative regulator of ChaA, a Na+/H+ antiporter that, also has Ca+/H+ activity in E. coli. ChaB contains a conserved 60-residue, region of unknown function found in other bacteria, archaeabacteria and a, series of baculoviral proteins. As part of a structural genomics project, the structure of ChaB was elucidated by NMR spectroscopy. RESULTS: The, structure of ChaB is composed of 3 alpha-helices and a small sheet that, pack tightly to form a fold that is found in the cyclin-box family of, proteins. CONCLUSION: ChaB is distinguished from its putative DNA binding, sequence homologues by a highly charged flexible loop region that has weak, affinity to Mg2+ and Ca2+ divalent metal ions.
<StructureSection load='1sg7' size='340' side='right'caption='[[1sg7]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sg7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SG7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sg7 OCA], [https://pdbe.org/1sg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1sg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sg7 ProSAT], [https://www.topsan.org/Proteins/BSGI/1sg7 TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHAB_ECO57 CHAB_ECO57] Might be a regulator of the sodium-potassium/proton antiporter ChaA.<ref>PMID:15306028</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/1sg7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sg7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: ChaB is a putative regulator of ChaA, a Na+/H+ antiporter that also has Ca+/H+ activity in E. coli. ChaB contains a conserved 60-residue region of unknown function found in other bacteria, archaeabacteria and a series of baculoviral proteins. As part of a structural genomics project, the structure of ChaB was elucidated by NMR spectroscopy. RESULTS: The structure of ChaB is composed of 3 alpha-helices and a small sheet that pack tightly to form a fold that is found in the cyclin-box family of proteins. CONCLUSION: ChaB is distinguished from its putative DNA binding sequence homologues by a highly charged flexible loop region that has weak affinity to Mg2+ and Ca2+ divalent metal ions.


==About this Structure==
The solution structure of ChaB, a putative membrane ion antiporter regulator from Escherichia coli.,Osborne MJ, Siddiqui N, Iannuzzi P, Gehring K BMC Struct Biol. 2004 Aug 11;4:9. PMID:15306028<ref>PMID:15306028</ref>
1SG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SG7 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The solution structure of ChaB, a putative membrane ion antiporter regulator from Escherichia coli., Osborne MJ, Siddiqui N, Iannuzzi P, Gehring K, BMC Struct Biol. 2004 Aug 11;4:9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15306028 15306028]
</div>
<div class="pdbe-citations 1sg7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: BSGI, Montreal-Kingston.Bacterial.Structural.Genomics.Initiative.]]
[[Category: Cygler M]]
[[Category: Cygler, M.]]
[[Category: Gehring K]]
[[Category: Gehring, K.]]
[[Category: Osborne MJ]]
[[Category: Osborne, M.J.]]
[[Category: Siddiqui N]]
[[Category: Siddiqui, N.]]
[[Category: bsgi]]
[[Category: montreal-kingston bacterial structural genomics initiative]]
[[Category: structural genomics]]
 
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Latest revision as of 09:08, 22 May 2024

NMR solution structure of the putative cation transport regulator ChaB

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