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New page: left|200px<br /><applet load="1smt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1smt, resolution 2.2Å" /> '''SMTB REPRESSOR FROM S...
 
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[[Image:1smt.gif|left|200px]]<br /><applet load="1smt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1smt, resolution 2.2&Aring;" />
'''SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942'''<br />


==Overview==
==SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942==
SmtB from Synechococcus PCC7942 is a trans-acting dimeric repressor that, is required for Zn(2+)-responsive expression of the metallothionein SmtA., The structure of SmtB was solved using multiple isomorphous replacement, techniques and refined at 2.2 A resolution by simulated annealing to an, R-factor of 0.218. SmtB displays the classical helix-turn-helix motif, found in many DNA-binding proteins. It has an alpha + beta topology, and, the arrangement of the three core helices and the beta hairpin is similar, to the HNF-3/fork head, CAP and diphtheria toxin repressor proteins., Although there is no zinc in the crystal structure, analysis of a mercuric, acetate derivative suggests a total of four Zn2+ binding sites in the, dimer. Two of these putative sites are at the opposite ends of the dimer, while the other two are at the dimer interface and are formed by residues, contributed from each monomer. The structure of the dimer is such that, simultaneous binding for both recognition helices to DNA would require, either a bend in the DNA helix or a conformational change in the dimer., The structure of Synechococcus SmtB is the first in this family of, metal-binding DNA repressors.
<StructureSection load='1smt' size='340' side='right'caption='[[1smt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1smt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SMT FirstGlance]. <br>
1SMT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smt OCA], [https://pdbe.org/1smt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1smt RCSB], [https://www.ebi.ac.uk/pdbsum/1smt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1smt ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the cyanobacterial metallothionein repressor SmtB: a model for metalloregulatory proteins., Cook WJ, Kar SR, Taylor KB, Hall LM, J Mol Biol. 1998 Jan 16;275(2):337-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466913 9466913]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/SMTB_SYNE7 SMTB_SYNE7] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:12146943</ref> <ref>PMID:12407207</ref> <ref>PMID:8871549</ref>
[[Category: Synechococcus sp.]]
== Evolutionary Conservation ==
[[Category: Cook, W.J.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Hall, L.M.]]
Check<jmol>
[[Category: dna repressor]]
  <jmolCheckbox>
[[Category: metalloregulation]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sm/1smt_consurf.spt"</scriptWhenChecked>
[[Category: zinc]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:29:55 2007''
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1smt ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
[[Category: Cook WJ]]
[[Category: Hall LM]]
Retrieved from "https://proteopedia.org/1smt"