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[[Image:1sro.gif|left|200px]]<br /><applet load="1sro" size="450" color="white" frame="true" align="right" spinBox="true"
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'''S1 RNA BINDING DOMAIN, NMR, 20 STRUCTURES'''<br />


==Overview==
==S1 RNA BINDING DOMAIN, NMR, 20 STRUCTURES==
The S1 domain, originally identified in ribosomal protein S1, is found in, a large number of RNA-associated proteins. The structure of the S1, RNA-binding domain from the E. coli polynucleotide phosphorylase has been, determined using NMR methods and consists of a five-stranded antiparallel, beta barrel. Conserved residues on one face of the barrel and adjacent, loops form the putative RNA-binding site. The structure of the S1 domain, is very similar to that of cold shock protein, suggesting that they are, both derived from an ancient nucleic acid-binding protein. Enhanced, sequence searches reveal hitherto unidentified S1 domains in RNase E, RNase II, NusA, EMB-5, and other proteins.
<StructureSection load='1sro' size='340' side='right'caption='[[1sro]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sro]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SRO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sro OCA], [https://pdbe.org/1sro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sro RCSB], [https://www.ebi.ac.uk/pdbsum/1sro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sro ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PNP_ECOLI PNP_ECOLI] Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction.[HAMAP-Rule:MF_01595]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sro_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sro ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The S1 domain, originally identified in ribosomal protein S1, is found in a large number of RNA-associated proteins. The structure of the S1 RNA-binding domain from the E. coli polynucleotide phosphorylase has been determined using NMR methods and consists of a five-stranded antiparallel beta barrel. Conserved residues on one face of the barrel and adjacent loops form the putative RNA-binding site. The structure of the S1 domain is very similar to that of cold shock protein, suggesting that they are both derived from an ancient nucleic acid-binding protein. Enhanced sequence searches reveal hitherto unidentified S1 domains in RNase E, RNase II, NusA, EMB-5, and other proteins.


==About this Structure==
The solution structure of the S1 RNA binding domain: a member of an ancient nucleic acid-binding fold.,Bycroft M, Hubbard TJ, Proctor M, Freund SM, Murzin AG Cell. 1997 Jan 24;88(2):235-42. PMID:9008164<ref>PMID:9008164</ref>
1SRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SRO OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The solution structure of the S1 RNA binding domain: a member of an ancient nucleic acid-binding fold., Bycroft M, Hubbard TJ, Proctor M, Freund SM, Murzin AG, Cell. 1997 Jan 24;88(2):235-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9008164 9008164]
</div>
<div class="pdbe-citations 1sro" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
*[[Ribosomal protein S1|Ribosomal protein S1]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bycroft, M.]]
[[Category: Bycroft M]]
[[Category: polynucleotide phosphorylase (pnpase)]]
[[Category: s1 rna-binding domain]]
 
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