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New page: left|200px<br /><applet load="1stz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1stz, resolution 2.20Å" /> '''Crystal structure of...
 
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[[Image:1stz.gif|left|200px]]<br /><applet load="1stz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1stz, resolution 2.20&Aring;" />
'''Crystal structure of a hypothetical protein at 2.2 A resolution'''<br />


==Overview==
==Crystal structure of a hypothetical protein at 2.2 A resolution==
All cells have a defense mechanism against a sudden heat-shock stress., Commonly, they express a set of proteins that protect cellular proteins, from being denatured by heat. Among them, GroE and DnaK chaperones are, representative defending systems, and their transcription is regulated by, a heat-shock repressor protein HrcA. HrcA repressor controls the, transcription of groE and dnaK operons by binding the palindromic CIRCE, element, presumably as a dimer, and the activity of HrcA repressor is, modulated by GroE chaperones. Here, we report the first crystal structure, of a heat-inducible transcriptional repressor, HrcA, from Thermotoga, maritima at 2.2A resolution. The Tm_HrcA protein crystallizes as a dimer., The monomer is composed of three domains: an N-terminal winged, helix-turn-helix domain (WH), a GAF-like domain, and an inserted, dimerizing domain (IDD). The IDD shows a unique structural fold with an, anti-parallel beta-sheet composed of three beta-strands sided by four, alpha-helices. The Tm_HrcA dimer structure is formed through hydrophobic, contact between the IDDs and a limited contact that involves conserved, residues between the GAF-like domains. In the overall dimer structure, the, two WH domains are exposed, but the conformation of these two domains, seems to be incompatible with DNA binding. We suggest that our structure, may represent an inactive form of the HrcA repressor. Structural, implication on how the inactive form of HrcA may be converted to the, active form by GroEL binding to a conserved C-terminal sequence region of, HrcA is discussed.
<StructureSection load='1stz' size='340' side='right'caption='[[1stz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1stz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STZ FirstGlance]. <br>
1STZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1STZ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1stz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1stz OCA], [https://pdbe.org/1stz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1stz RCSB], [https://www.ebi.ac.uk/pdbsum/1stz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1stz ProSAT], [https://www.topsan.org/Proteins/BSGC/1stz TOPSAN]</span></td></tr>
==Reference==
</table>
Crystal structure of a heat-inducible transcriptional repressor HrcA from Thermotoga maritima: structural insight into DNA binding and dimerization., Liu J, Huang C, Shin DH, Yokota H, Jancarik J, Kim JS, Adams PD, Kim R, Kim SH, J Mol Biol. 2005 Jul 29;350(5):987-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15979091 15979091]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/HRCA_THEMA HRCA_THEMA] Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/st/1stz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1stz ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Adams, P.D.]]
[[Category: Adams PD]]
[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
[[Category: Huang C]]
[[Category: Huang, C.]]
[[Category: Jancarik J]]
[[Category: Jancarik, J.]]
[[Category: Kim R]]
[[Category: Kim, R.]]
[[Category: Kim S-H]]
[[Category: Kim, S.H.]]
[[Category: Liu J]]
[[Category: Liu, J.]]
[[Category: Shin D-H]]
[[Category: Shin, D.H.]]
[[Category: Yokota H]]
[[Category: Yokota, H.]]
[[Category: berkeley structural genomics center]]
[[Category: bsgc structure funded by nih]]
[[Category: circe element]]
[[Category: hrca]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomics]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:39:56 2007''
Retrieved from "https://proteopedia.org/1stz"