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New page: left|200px<br /><applet load="1sxm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sxm" /> '''SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFIN...
 
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[[Image:1sxm.gif|left|200px]]<br /><applet load="1sxm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sxm" />
'''SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)'''<br />


==Overview==
==SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)==
The 3D structure of noxiustoxin, the first identified scorpion toxin, acting on K+ channels, has been elucidated by NMR and molecular modeling., Thirty-nine solution structures were calculated using 572 distance and 42, dihedral restraints. The average atomic rms deviation between the refined, structures and the mean structure is 0.75 A for the backbone atoms., Noxiustoxin adopts a alpha/beta scaffold constituted of a three-stranded, beta-sheet (residues 2-3, 25-30, 33-38) linked to a helix (residues 10-20), through two disulfide bridges. A comparison between the 3D structure of, noxiustoxin and those of other structurally and functionally related, scorpion toxins (charybdotoxin, PO5-NH2, kaliotoxin) revealed a bending, capacity of the helix and a variability in the relative orientations, between the helix and the beta-sheet. These two features highlight the, plasticity of the alpha/beta scaffold and offer a structural explanation, for the capacity of the fold to accommodate an additional alanine residue, in the Gly-x-Cys pattern of a previously proposed consensus sequence, [Bontems et al. (1991) Science 254, 1521-1523]. Our structural data also, emphasize the possibility that the beta-sheet of NTX is implicated in the, capacity of NTX to recognize voltage-dependent K+ channels.
<StructureSection load='1sxm' size='340' side='right'caption='[[1sxm]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1sxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxm OCA], [https://pdbe.org/1sxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxm RCSB], [https://www.ebi.ac.uk/pdbsum/1sxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KAX21_CENNO KAX21_CENNO] Blocks voltage-gated non-inactivating potassium channels and unblocks inactivating potassium channels blocked by alpha-dendrotoxin in synaptosomes. Also displaces the alpha-dendrotoxin homolog dendrotoxin I from its receptor on brain synaptic membranes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1SXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA].
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Determination of the three-dimensional solution structure of noxiustoxin: analysis of structural differences with related short-chain scorpion toxins., Dauplais M, Gilquin B, Possani LD, Gurrola-Briones G, Roumestand C, Menez A, Biochemistry. 1995 Dec 26;34(51):16563-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8527429 8527429]
[[Category: Centruroides noxius]]
[[Category: Centruroides noxius]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dauplais, M.]]
[[Category: Dauplais M]]
[[Category: Gilquin, B.]]
[[Category: Gilquin B]]
[[Category: Gurrola-Briones, G.]]
[[Category: Gurrola-Briones G]]
[[Category: Menez, A.]]
[[Category: Menez A]]
[[Category: Possani, L.D.]]
[[Category: Possani LD]]
[[Category: Roumestand, C.]]
[[Category: Roumestand C]]
[[Category: NH2]]
[[Category: toxin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:49:24 2007''
Retrieved from "https://proteopedia.org/1sxm"