1t01: Difference between revisions

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New page: left|200px<br /><applet load="1t01" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t01, resolution 2.06Å" /> '''Vinculin complexed w...
 
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[[Image:1t01.gif|left|200px]]<br /><applet load="1t01" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t01, resolution 2.06&Aring;" />
'''Vinculin complexed with the VBS1 helix from talin'''<br />


==Overview==
==Vinculin complexed with the VBS1 helix from talin==
The interaction between the cytoskeletal proteins talin and vinculin plays, a key role in integrin-mediated cell adhesion and migration. We have, determined the crystal structures of two domains from the talin rod, spanning residues 482-789. Talin 482-655, which contains a, vinculin-binding site (VBS), folds into a five-helix bundle whereas talin, 656-789 is a four-helix bundle. We show that the VBS is composed of a, hydrophobic surface spanning five turns of helix 4. All the key side, chains from the VBS are buried and contribute to the hydrophobic core of, the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix, bundle represents an inactive conformation, and mutations that disrupt the, hydrophobic core or deletion of helix 5 are required to induce an active, conformation in which the VBS is exposed. We also report the crystal, structure of the N-terminal vinculin head domain in complex with an, activated form of talin. Activation of the VBS in talin and the, recruitment of vinculin may support the maturation of small integrin/talin, complexes into more stable adhesions.
<StructureSection load='1t01' size='340' side='right'caption='[[1t01]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1t01]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T01 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t01 OCA], [https://pdbe.org/1t01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t01 RCSB], [https://www.ebi.ac.uk/pdbsum/1t01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t01 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VINC_CHICK VINC_CHICK] Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.<ref>PMID:15229287</ref> <ref>PMID:20584916</ref> <ref>PMID:20086044</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t01_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t01 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1T01 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T01 OCA].
*[[Isocitrate dehydrogenase|Isocitrate dehydrogenase]]
 
*[[Talin 3D structures|Talin 3D structures]]
==Reference==
*[[Vinculin|Vinculin]]
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272303 15272303]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Barsukov IL]]
[[Category: Barsukov, I.L.]]
[[Category: Critchley DR]]
[[Category: Critchley, D.R.]]
[[Category: Emsley J]]
[[Category: Emsley, J.]]
[[Category: Gingras AR]]
[[Category: Gingras, A.R.]]
[[Category: Papagrigoriou E]]
[[Category: Papagrigoriou, E.]]
[[Category: five helix bundle]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:53:13 2007''
Retrieved from "https://proteopedia.org/1t01"