1t16: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1t16" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t16, resolution 2.60Å" /> '''Crystal structure of...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1t16.gif|left|200px]]<br /><applet load="1t16" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t16, resolution 2.60&Aring;" />
'''Crystal structure of the bacterial fatty acid transporter FadL from Escherichia coli'''<br />


==Overview==
==Crystal structure of the bacterial fatty acid transporter FadL from Escherichia coli==
The mechanisms by which hydrophobic molecules, such as long-chain fatty, acids, enter cells are poorly understood. In Gram-negative bacteria, the, lipopolysaccharide layer in the outer membrane is an efficient barrier for, fatty acids and aromatic hydrocarbons destined for biodegradation. We, report crystal structures of the long-chain fatty acid transporter FadL, from Escherichia coli at 2.6 and 2.8 angstrom resolution. FadL forms a, 14-stranded beta barrel that is occluded by a central hatch domain. The, structures suggest that hydrophobic compounds bind to multiple sites in, FadL and use a transport mechanism that involves spontaneous, conformational changes in the hatch.
<StructureSection load='1t16' size='340' side='right'caption='[[1t16]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1t16]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T16 FirstGlance]. <br>
1T16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CU, LDA and C8E as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T16 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t16 OCA], [https://pdbe.org/1t16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t16 RCSB], [https://www.ebi.ac.uk/pdbsum/1t16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t16 ProSAT]</span></td></tr>
Crystal structure of the long-chain fatty acid transporter FadL., van den Berg B, Black PN, Clemons WM Jr, Rapoport TA, Science. 2004 Jun 4;304(5676):1506-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15178802 15178802]
</table>
== Function ==
[https://www.uniprot.org/uniprot/FADL_ECOLI FADL_ECOLI] Involved in translocation of long-chain fatty acids across the outer membrane. It is a receptor for the bacteriophage T2. FadL may form a specific channel.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t16_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t16 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berg, B.van.den.]]
[[Category: Black PN]]
[[Category: Black, P.N.]]
[[Category: Clemons Jr WM]]
[[Category: Jr., W.M.Clemons.]]
[[Category: Rapoport TA]]
[[Category: Rapoport, T.A.]]
[[Category: Van den Berg B]]
[[Category: C8E]]
[[Category: CU]]
[[Category: LDA]]
[[Category: beta-barrel]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:54:49 2007''
Retrieved from "https://proteopedia.org/1t16"