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New page: left|200px<br /><applet load="1tcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tcs, resolution 1.7Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1tcs.jpg|left|200px]]<br /><applet load="1tcs" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tcs, resolution 1.7&Aring;" />
'''CRYSTAL STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROMS RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROMS RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE==
We describe here the crystal structure of the trichosanthin-NADPH complex, determined at a resolution of 1.7 A. The adenine base stacks between Tyr, 70 and Tyr 111. Arg 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and, through a water molecule, to N(9) of adenosine, respectively., This is the first high resolution structure of a complex between a, ribosome-inactivating protein and a substrate analogue, in which the, electron density of the N-glycosidic bond is well defined and the, preassociated water, thought to be responsible for hydrolyzing the N-C, bond, is also explicitly elucidated.
<StructureSection load='1tcs' size='340' side='right'caption='[[1tcs]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tcs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichosanthes_kirilowii Trichosanthes kirilowii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCS FirstGlance]. <br>
1TCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichosanthes_kirilowii Trichosanthes kirilowii] with NDP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TCS OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tcs OCA], [https://pdbe.org/1tcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tcs RCSB], [https://www.ebi.ac.uk/pdbsum/1tcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tcs ProSAT]</span></td></tr>
Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture., Xiong JP, Xia ZX, Wang Y, Nat Struct Biol. 1994 Oct;1(10):695-700. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7634073 7634073]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/RIPT_TRIKI RIPT_TRIKI] Inactivates eukaryotic 60S ribosomal subunits.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tcs_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tcs ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trichosanthes kirilowii]]
[[Category: Trichosanthes kirilowii]]
[[Category: rRNA N-glycosylase]]
[[Category: Wang Y]]
[[Category: Wang, Y.]]
[[Category: Xia Z-X]]
[[Category: Xia, Z.X.]]
[[Category: Xiong J-P]]
[[Category: Xiong, J.P.]]
[[Category: NDP]]
[[Category: toxin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:09:37 2007''

Latest revision as of 08:38, 14 February 2024

CRYSTAL STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROMS RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE

1tcs, resolution 1.70Å

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