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New page: left|200px<br /><applet load="1tfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tfu, resolution 1.99Å" /> '''phosphopantetheine a...
 
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[[Image:1tfu.gif|left|200px]]<br /><applet load="1tfu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tfu, resolution 1.99&Aring;" />
'''phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis'''<br />


==Overview==
==phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis==
Phosphopantetheine adenylyltransferase (PPAT) catalyzes the penultimate, step in prokaryotic coenzyme A (CoA) biosynthesis, directing the transfer, of an adenylyl group from ATP to 4'-phosphopantetheine (Ppant) to yield, dephospho-CoA (dPCoA). The crystal structures of Escherichia coli PPAT, bound to its substrates, product, and inhibitor revealed an allosteric, hexameric enzyme with half-of-sites reactivity, and established an in-line, displacement catalytic mechanism. To provide insight into the mechanism of, ligand binding we solved the apoenzyme (Apo) crystal structure of PPAT, from Mycobacterium tuberculosis. In its Apo form, PPAT is a symmetric, hexamer with an open solvent channel. However, ligand binding provokes, asymmetry and alters the structure of the solvent channel, so that ligand, binding becomes restricted to one trimer.
<StructureSection load='1tfu' size='340' side='right'caption='[[1tfu]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tfu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TFU FirstGlance]. <br>
1TFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Pantetheine-phosphate_adenylyltransferase Pantetheine-phosphate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.3 2.7.7.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TFU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tfu OCA], [https://pdbe.org/1tfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tfu RCSB], [https://www.ebi.ac.uk/pdbsum/1tfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tfu ProSAT]</span></td></tr>
==Reference==
</table>
Substrate-induced asymmetry and channel closure revealed by the apoenzyme structure of Mycobacterium tuberculosis phosphopantetheine adenylyltransferase., Morris VK, Izard T, Protein Sci. 2004 Sep;13(9):2547-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15322293 15322293]
== Function ==
[https://www.uniprot.org/uniprot/COAD_MYCTU COAD_MYCTU] Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tf/1tfu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tfu ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Pantetheine-phosphate adenylyltransferase]]
[[Category: Izard T]]
[[Category: Single protein]]
[[Category: Morris VK]]
[[Category: Izard, T.]]
[[Category: Morris, V.K.]]
[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomics]]
[[Category: tb structural genomics consortium]]
[[Category: tbsgc]]
[[Category: transport protein]]
 
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Retrieved from "https://proteopedia.org/1tfu"