2w1t: Difference between revisions
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New page: '''Unreleased structure''' The entry 2w1t is ON HOLD Authors: Asen, I., Djuranovic, S., Lupas, A.N., Zeth, K. Description: Crystal Structure of B. subtilis SpoVT ''Page seeded by [htt... |
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==Crystal Structure of B. subtilis SpoVT== | |||
<StructureSection load='2w1t' size='340' side='right'caption='[[2w1t]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2w1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1t OCA], [https://pdbe.org/2w1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1t RCSB], [https://www.ebi.ac.uk/pdbsum/2w1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SPOVT_BACSU SPOVT_BACSU] Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).<ref>PMID:22522895</ref> <ref>PMID:8755877</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the crystal structure of the isolated C-terminal domain of SpoVT at 1.5 A by experimental phasing techniques and used this model to solve the structure of the full-length SpoVT at 2.6 A by molecular replacement. SpoVT is a tetramer that shows an overall significant distortion mediated by electrostatic interactions. Two monomers dimerize via the highly charged N-terminal domains to form swapped-hairpin beta-barrels. These asymmetric dimers further tetramerize through the formation of mixed helix bundles between their C-terminal domains, which themselves fold as GAF (cGMP-specific and cGMP-stimulated phosphodiesterases, Anabaena adenylate cyclases, and Escherichia coli FhlA) domains. The combination of a swapped-hairpin beta-barrel with a GAF domain represents a novel domain architecture in transcription factors. The occurrence of SpoVT homologs throughout Bacilli and Clostridia demonstrates the ancestral origin of this factor in sporulation. | |||
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis.,Asen I, Djuranovic S, Lupas AN, Zeth K J Mol Biol. 2008 Oct 30. PMID:18996130<ref>PMID:18996130</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2w1t" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus subtilis]] | |||
[[Category: Large Structures]] | |||
[[Category: Asen I]] | |||
[[Category: Djuranovic S]] | |||
[[Category: Lupas AN]] | |||
[[Category: Zeth K]] | |||
Latest revision as of 07:16, 9 August 2023
Crystal Structure of B. subtilis SpoVT
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