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New page: left|200px<br /><applet load="1tlh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tlh" /> '''T4 AsiA bound to sigma70 region 4'''<br /> ...
 
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[[Image:1tlh.jpg|left|200px]]<br /><applet load="1tlh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tlh" />
'''T4 AsiA bound to sigma70 region 4'''<br />


==Overview==
==T4 AsiA bound to sigma70 region 4==
Bacteriophage T4 AsiA is a versatile transcription factor capable of, inhibiting host gene expression as an 'anti-sigma' factor while, simultaneously promoting gene-specific expression of T4 middle genes in, conjunction with T4 MotA. To accomplish this task, AsiA engages conserved, region 4 of Eschericia coli sigma70, blocking recognition of most host, promoters by sequestering the DNA-binding surface at the AsiA/sigma70, interface. The three-dimensional structure of an AsiA/region 4 complex, reveals that the C-terminal alpha helix of region 4 is unstructured, while, four other helices adopt a completely different conformation relative to, the canonical structure of unbound region 4. That AsiA induces, rather, than merely stabilizes, this rearrangement can be realized by comparison, to the homologous structures of region 4 solved in a variety of contexts, including the structure of Thermotoga maritima sigmaA region 4 described, herein. AsiA simultaneously occupies the surface of region 4 that, ordinarily contacts core RNA polymerase (RNAP), suggesting that an, AsiA-bound sigma70 may also undergo conformational changes in the context, of the RNAP holoenzyme.
<StructureSection load='1tlh' size='340' side='right'caption='[[1tlh]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1tlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlh OCA], [https://pdbe.org/1tlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tlh RCSB], [https://www.ebi.ac.uk/pdbsum/1tlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tlh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tlh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tlh ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1TLH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLH OCA].
*[[Sigma factor 3D structures|Sigma factor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
T4 AsiA blocks DNA recognition by remodeling sigma70 region 4., Lambert LJ, Wei Y, Schirf V, Demeler B, Werner MH, EMBO J. 2004 Aug 4;23(15):2952-62. Epub 2004 Jul 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15257291 15257291]
[[Category: Bacteriophage t4]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Escherichia virus T4]]
[[Category: Demeler, B.]]
[[Category: Large Structures]]
[[Category: Lambert, L.J.]]
[[Category: Demeler B]]
[[Category: Schirf, V.]]
[[Category: Lambert LJ]]
[[Category: Wei, Y.]]
[[Category: Schirf V]]
[[Category: Werner, M.H.]]
[[Category: Wei Y]]
[[Category: anti-sigma]]
[[Category: Werner MH]]
[[Category: nmr]]
[[Category: rna polymerase]]
[[Category: sigma70]]
 
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