2e28: Difference between revisions

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{{Seed}}
[[Image:2e28.png|left|200px]]


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==Crystal structure analysis of pyruvate kinase from Bacillus stearothermophilus==
The line below this paragraph, containing "STRUCTURE_2e28", creates the "Structure Box" on the page.
<StructureSection load='2e28' size='340' side='right'caption='[[2e28]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2e28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E28 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_2e28|  PDB=2e28  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e28 OCA], [https://pdbe.org/2e28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e28 RCSB], [https://www.ebi.ac.uk/pdbsum/2e28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e28 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KPYK_GEOSE KPYK_GEOSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/2e28_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e28 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The pyruvate kinase (PK) from a moderate thermophile, Geobacillus stearothermophilus, is an allosteric enzyme activated by AMP and ribose 5-phosphate but not fructose 1, 6-bisphosphate (FBP), which is a common activator of PKs. It has an extra C-terminal sequence (ECTS), which contains a highly conserved phosphoenolpyruvate (PEP) binding motif, but its function and structure remain unclear. To elucidate the structural characteristics of the effector-binding site and the ECTS, the crystal structure of the C9S/C268S mutant of the enzyme was determined at 2.4 A resolution. The crystal belonged to space group P6(2)22, with unit cell parameters a, b = 145.97 A, c = 118.03 A. The enzyme was a homotetramer and its overall domain structure was similar to the previously solved structures except that the ECTS formed a new domain (C' domain). The structure of the C' domain closely resembled that of the PEP binding domain of maize pyruvate phosphate dikinase. A sulphate ion was found in a pocket in the effector-binding C domain. This site corresponds to the 6-phosphate group-binding site in yeast PK bound FBP and seems to be the effector-binding site. Through comparison of the structure of the putative effector-binding site to that of the FBP binding site of the yeast enzyme, the structural basis of the effector specificity of the G. stearothermophilus PK is discussed.


===Crystal structure analysis of pyruvate kinase from Bacillus stearothermophilus===
Crystal structure of pyruvate kinase from Geobacillus stearothermophilus.,Suzuki K, Ito S, Shimizu-Ibuka A, Sakai H J Biochem. 2008 Sep;144(3):305-12. Epub 2008 May 28. PMID:18511452<ref>PMID:18511452</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2e28" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18511452}}, adds the Publication Abstract to the page
*[[Pyruvate kinase 3D structures|Pyruvate kinase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18511452 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_18511452}}
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</StructureSection>
==About this Structure==
2E28 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E28 OCA].
 
==Reference==
Crystal structure of pyruvate kinase from Geobacillus stearothermophilus., Suzuki K, Ito S, Shimizu-Ibuka A, Sakai H, J Biochem. 2008 Sep;144(3):305-12. Epub 2008 May 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18511452 18511452]
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Pyruvate kinase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Suzuki K]]
[[Category: Suzuki, K.]]
[[Category: Allosteric]]
[[Category: Pyruvate kinase]]
[[Category: Transferase]]
 
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Retrieved from "https://proteopedia.org/2e28"