3e2e: Difference between revisions

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{{Seed}}
[[Image:3e2e.jpg|left|200px]]


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==Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA==
The line below this paragraph, containing "STRUCTURE_3e2e", creates the "Structure Box" on the page.
<StructureSection load='3e2e' size='340' side='right'caption='[[3e2e]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3e2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E2E FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e2e OCA], [https://pdbe.org/3e2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e2e RCSB], [https://www.ebi.ac.uk/pdbsum/3e2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e2e ProSAT]</span></td></tr>
{{STRUCTURE_3e2e| PDB=3e2e |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e2/3e2e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e2e ConSurf].
<div style="clear:both"></div>


===Crystal Structure of an Intermediate Complex of T7 RNAP and 7nt of RNA===
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 
__TOC__
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</StructureSection>
The line below this paragraph, {{ABSTRACT_PUBMED_18948533}}, adds the Publication Abstract to the page
[[Category: Escherichia phage T7]]
(as it appears on PubMed at http://www.pubmed.gov), where 18948533 is the PubMed ID number.
[[Category: Large Structures]]
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[[Category: Synthetic construct]]
{{ABSTRACT_PUBMED_18948533}}
[[Category: Bailey S]]
 
[[Category: Durniak KJ]]
==About this Structure==
[[Category: Steitz TA]]
3E2E is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E2E OCA].
 
==Reference==
The structure of a transcribing t7 RNA polymerase in transition from initiation to elongation., Durniak KJ, Bailey S, Steitz TA, Science. 2008 Oct 24;322(5901):553-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18948533 18948533]
[[Category: DNA-directed RNA polymerase]]
[[Category: Enterobacteria phage t7]]
[[Category: Protein complex]]
[[Category: Bailey, S.]]
[[Category: Durniak, K J.]]
[[Category: Steitz, T A.]]
[[Category: Dna-directed rna polymerase]]
[[Category: Nucleotidyltransferase]]
[[Category: T7 rna polymerase]]
[[Category: Transcription]]
[[Category: Transferase]]
[[Category: Transferase/rna/dna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov  5 12:51:01 2008''
Retrieved from "https://proteopedia.org/3e2e"