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New page: left|200px<br /><applet load="1tro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tro, resolution 1.900Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1tro.gif|left|200px]]<br /><applet load="1tro" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tro, resolution 1.900&Aring;" />
'''CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION'''<br />


==Overview==
==CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION==
The crystal structure of the trp repressor/operator complex shows an, extensive contact surface, including 24 direct and 6 solvent-mediated, hydrogen bonds to the phosphate groups of the DNA. There are no direct, hydrogen bonds or non-polar contacts to the bases that can explain the, repressor's specificity for the operator sequence. Rather, the sequence, seems to be recognized indirectly through its effects on the geometry of, the phosphate backbone, which in turn permits the formation of a stable, interface. Water-mediated polar contacts to the bases also appear to, contribute part of the specificity.
<StructureSection load='1tro' size='340' side='right'caption='[[1tro]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tro]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TRO FirstGlance]. <br>
1TRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TRO OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tro OCA], [https://pdbe.org/1tro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tro RCSB], [https://www.ebi.ac.uk/pdbsum/1tro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tro ProSAT]</span></td></tr>
Crystal structure of trp repressor/operator complex at atomic resolution., Otwinowski Z, Schevitz RW, Zhang RG, Lawson CL, Joachimiak A, Marmorstein RQ, Luisi BF, Sigler PB, Nature. 1988 Sep 22;335(6188):321-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3419502 3419502]
</table>
[[Category: Escherichia coli]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/TRPR_ECOLI TRPR_ECOLI] This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.
[[Category: Joachimiak, A.J.]]
== Evolutionary Conservation ==
[[Category: Lawson, C.L.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Luisi, B.F.]]
Check<jmol>
[[Category: Marmorstein, R.]]
  <jmolCheckbox>
[[Category: Otwinowski, Z.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tr/1tro_consurf.spt"</scriptWhenChecked>
[[Category: Schevitz, R.W.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Sigler, P.B.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Zhang, R.G.]]
  </jmolCheckbox>
[[Category: TRP]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tro ConSurf].
[[Category: double helix]]
<div style="clear:both"></div>
[[Category: protein-dna complex]]
__TOC__
 
</StructureSection>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:32:35 2007''
[[Category: Escherichia coli str. K-12 substr. W3110]]
[[Category: Large Structures]]
[[Category: Joachimiak A]]
[[Category: Lawson CL]]
[[Category: Luisi BF]]
[[Category: Marmorstein R]]
[[Category: Otwinowski Z]]
[[Category: Schevitz RW]]
[[Category: Sigler PB]]
[[Category: Zhang R-G]]

Latest revision as of 08:41, 14 February 2024

CRYSTAL STRUCTURE OF TRP REPRESSOR OPERATOR COMPLEX AT ATOMIC RESOLUTION

1tro, resolution 1.90Å

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