3eut: Difference between revisions

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{{Seed}}
[[Image:3eut.jpg|left|200px]]


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==X-ray crystal structure of a type III pentaketide synthase from Neurospora crassa==
The line below this paragraph, containing "STRUCTURE_3eut", creates the "Structure Box" on the page.
<StructureSection load='3eut' size='340' side='right'caption='[[3eut]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3eut]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EUT FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=DCR:ICOSANOIC+ACID'>DCR</scene></td></tr>
{{STRUCTURE_3eut|  PDB=3eut  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eut FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eut OCA], [https://pdbe.org/3eut PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eut RCSB], [https://www.ebi.ac.uk/pdbsum/3eut PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eut ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7S6N4_NEUCR Q7S6N4_NEUCR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/3eut_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eut ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The fungal type III polyketide synthase 2'-oxoalkylresorcylic acid synthase (ORAS) primes with a range of acyl-Coenzyme A thioesters (C4-C20) and extends using malonyl-Coenzyme A to produce pyrones, resorcinols, and resorcylic acids. To gain insight into this unusual substrate specificity and product profile, we have determined the crystal structures of ORAS to 1.75 A resolution, the Phe-252--&gt;Gly site-directed mutant to 2.1 A resolution, and a binary complex of ORAS with eicosanoic acid to 2.0 A resolution. The structures reveal a distinct rearrangement of structural elements near the active site that allows accommodation of long-chain fatty acid esters and a reorientation of the gating mechanism that controls cyclization and polyketide chain length. The roles of these structural elements are further elucidated by characterization of various structure-based site-directed variants. These studies establish an unexpected plasticity to the PKS fold, unanticipated from structural studies of other members of this enzyme family.


===X-ray crystal structure of a type III pentaketide synthase from Neurospora crassa===
Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa.,Rubin-Pitel SB, Zhang H, Vu T, Brunzelle JS, Zhao H, Nair SK Chem Biol. 2008 Oct 20;15(10):1079-90. PMID:18940668<ref>PMID:18940668</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_18940668}}, adds the Publication Abstract to the page
<div class="pdbe-citations 3eut" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 18940668 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18940668}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
3EUT is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EUT OCA].
 
==Reference==
Distinct Structural Elements Dictate the Specificity of the Type III Pentaketide Synthase from Neurospora crassa., Rubin-Pitel SB, Zhang H, Vu T, Brunzelle JS, Zhao H, Nair SK, Chem Biol. 2008 Oct 20;15(10):1079-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18940668 18940668]
[[Category: Neurospora crassa]]
[[Category: Neurospora crassa]]
[[Category: Brunzelle, J S.]]
[[Category: Brunzelle JS]]
[[Category: Nair, S K.]]
[[Category: Nair SK]]
[[Category: Zhang, H.]]
[[Category: Zhang H]]
[[Category: Acyltransferase]]
[[Category: Ora]]
[[Category: Transferase]]
 
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Retrieved from "https://proteopedia.org/3eut"