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New page: left|200px<br /><applet load="1tul" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tul, resolution 2.2Å" /> '''STRUCTURE OF TLP20'''...
 
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[[Image:1tul.gif|left|200px]]<br /><applet load="1tul" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1tul, resolution 2.2&Aring;" />
'''STRUCTURE OF TLP20'''<br />


==Overview==
==STRUCTURE OF TLP20==
Myosin light-chain kinase is responsible for the phosphorylation of myosin, in smooth muscle cells. In some tissue types, the C-terminal portion of, this large enzyme is expressed as an independent protein and has been, given the name telokin. Recently, an antibody directed against telokin was, found to interact with a protein derived from the baculovirus Autographa, californica nuclear polyhedrosis virus. This protein was biochemically, characterized and given the name TLP20 for telokin-like protein of 20 000, molecular weight. The amino-acid sequence of TLP20 was determined on the, basis of a cDNA clone and subsequent alignment searches failed to reveal, any homology to telokin or to other known proteins. The three-dimensional, structure of a proteolytic portion of TLP20 is reported here. Crystals, employed in the investigation were grown from ammonium sulfate solutions, at pH 6.0 and belonged to the space group P2(1)3 with unit-cell dimensions, of a = b = c = 76.3 A and one molecule per asymmetric unit. The structure, was determined by multiple isomorphous replacement with three heavy-atom, derivatives. Least-squares refinement of the model reduced the, crystallographic R factor to 18.1% for all measured X-ray data from 30.0, to 2.2 A. The overall fold of the molecule may be described as a, seven-stranded antiparallel beta-barrel flanked on the bottom by two, additional beta-strands and on the top by an alpha-helix. Quite, surprisingly, the three-dimensional structure of this beta-barrel is not, similar to telokin or to any other known protein.
<StructureSection load='1tul' size='340' side='right'caption='[[1tul]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1tul]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TUL FirstGlance]. <br>
1TUL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TUL OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tul OCA], [https://pdbe.org/1tul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tul RCSB], [https://www.ebi.ac.uk/pdbsum/1tul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tul ProSAT]</span></td></tr>
==Reference==
</table>
Molecular structure of a proteolytic fragment of TLP20., Holden HM, Wesenberg G, Raynes DA, Hartshorne DJ, Guerriero V Jr, Rayment I, Acta Crystallogr D Biol Crystallogr. 1996 Nov 1;52(Pt 6):1153-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299576 15299576]
== Function ==
[https://www.uniprot.org/uniprot/TLP20_NPVAC TLP20_NPVAC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tu/1tul_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tul ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Autographa californica nucleopolyhedrovirus]]
[[Category: Autographa californica nucleopolyhedrovirus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Holden, H.M.]]
[[Category: Holden HM]]
[[Category: Rayment, I.]]
[[Category: Rayment I]]
[[Category: telokin-like protein]]
 
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