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New page: left|200px<br /><applet load="1u0l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u0l, resolution 2.80Å" /> '''Crystal structure of...
 
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[[Image:1u0l.jpg|left|200px]]<br /><applet load="1u0l" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u0l, resolution 2.80&Aring;" />
'''Crystal structure of YjeQ from Thermotoga maritima'''<br />


==Overview==
==Crystal structure of YjeQ from Thermotoga maritima==
We have determined the crystal structure of the GDP complex of the YjeQ, protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase, subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known, to bind to the ribosome, is composed of three domains: an N-terminal, oligonucleotide/oligosaccharide-binding fold domain, a central GTPase, domain, and a C-terminal zinc-finger domain. The crystal structure of, TmYjeQ reveals two interesting domains: a circularly permutated GTPase, domain and an unusual zinc-finger domain. The binding mode of GDP in the, GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras, proteins, a prototype GTPase. The N-terminal, oligonucleotide/oligosaccharide-binding fold domain, together with the, GTPase domain, forms the extended RNA-binding site. The C-terminal domain, has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a, DNA-repair protein, rad51 fragment. The overall structural features of, TmYjeQ make it a good candidate for an RNA-binding protein, which is, consistent with the biochemical data of the YjeQ subfamily in binding to, the ribosome.
<StructureSection load='1u0l' size='340' side='right'caption='[[1u0l]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1u0l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0L FirstGlance]. <br>
1U0L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ZN and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U0L OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0l OCA], [https://pdbe.org/1u0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0l RCSB], [https://www.ebi.ac.uk/pdbsum/1u0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0l ProSAT], [https://www.topsan.org/Proteins/BSGC/1u0l TOPSAN]</span></td></tr>
Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain., Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13198-203. Epub 2004 Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15331784 15331784]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/RSGA_THEMA RSGA_THEMA] May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover.[HAMAP-Rule:MF_01820]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0l ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
[[Category: Jaru J]]
[[Category: Jaru, J.]]
[[Category: Kim R]]
[[Category: Kim, R.]]
[[Category: Kim SH]]
[[Category: Kim, S.H.]]
[[Category: Lou Y]]
[[Category: Lou, Y.]]
[[Category: Shin DH]]
[[Category: Shin, D.H.]]
[[Category: Yokota H]]
[[Category: Yokota, H.]]
[[Category: GDP]]
[[Category: ZN]]
[[Category: berkeley structural genomics center]]
[[Category: bsgc structure funded by nih]]
[[Category: gtpase]]
[[Category: ob-fold]]
[[Category: permutation]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomics]]
[[Category: zinc-finger]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:45:06 2007''
Retrieved from "https://proteopedia.org/1u0l"