1u2v: Difference between revisions

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New page: left|200px<br /><applet load="1u2v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u2v, resolution 2.55Å" /> '''Crystal structure of...
 
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[[Image:1u2v.jpg|left|200px]]<br /><applet load="1u2v" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u2v, resolution 2.55&Aring;" />
'''Crystal structure of Arp2/3 complex with bound ADP and calcium'''<br />


==Overview==
==Crystal structure of Arp2/3 complex with bound ADP and calcium==
Actin-related protein (Arp) 2/3 complex stimulates formation of actin, filaments at the leading edge of motile cells. Nucleation of filaments, depends on hydrolysis of ATP bound to Arp2. Here we report crystal, structures of Arp2/3 complex with bound ATP or ADP. The nucleotides are, immobilized on the face of subdomains 3 and 4 of Arp2, whereas subdomains, 1 and 2 are flexible and absent from the electron density maps. This, flexibility may explain why Arp2 does not hydrolyze ATP until the complex, is activated. ATP stabilizes a relatively closed conformation of Arp3 with, the gamma-phosphate bridging loops from opposite sides of the cleft. ADP, binds Arp3 in a unique conformation that favors an open cleft, revealing a, conformational change that may occur in actin and Arps when ATP is, hydrolyzed and phosphate dissociates. These structures provide the an, opportunity to compare all nucleotide-binding states in an actin-related, protein and give insights into the function of both the Arp2/3 complex and, actin.
<StructureSection load='1u2v' size='340' side='right'caption='[[1u2v]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1u2v]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U2V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u2v OCA], [https://pdbe.org/1u2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u2v RCSB], [https://www.ebi.ac.uk/pdbsum/1u2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u2v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u2/1u2v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u2v ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1U2V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U2V OCA].
*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP., Nolen BJ, Littlefield RS, Pollard TD, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15505213 15505213]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Littlefield, R.S.]]
[[Category: Littlefield RS]]
[[Category: Nolen, B.J.]]
[[Category: Nolen BJ]]
[[Category: Pollard, T.D.]]
[[Category: Pollard TD]]
[[Category: ADP]]
[[Category: CA]]
[[Category: structural protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:48:34 2007''

Latest revision as of 13:30, 13 March 2024

Crystal structure of Arp2/3 complex with bound ADP and calcium

1u2v, resolution 2.55Å

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