1u5o: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
New page: left|200px<br /><applet load="1u5o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u5o, resolution 2.50Å" /> '''Structure of the D23...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1u5o.jpg|left|200px]]<br /><applet load="1u5o" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u5o, resolution 2.50&Aring;" />
'''Structure of the D23A mutant of the nuclear transport carrier NTF2'''<br />


==Overview==
==Structure of the D23A mutant of the nuclear transport carrier NTF2==
Nuclear transport carriers interact with proteins of the nuclear pore, complex (NPC) to transport their cargo across the nuclear envelope. One, such carrier is nuclear transport factor 2 (NTF2), whose import cargo is, the small GTPase Ran. A domain highly homologous to the small NTF2 protein, (14kDa) is also found in a number of additional proteins, which together, make up the NTF2 domain containing superfamily of proteins. Using, structural, computational and biochemical analysis we have identified a, functional site that is present throughout this superfamily, and our, results indicate that this site functions as an NPC binding site in NTF2., Previously we showed that a D23A mutant of NTF2 exhibits increased, affinity for the NPC. The mechanism of this mutation, however, was unknown, as this region of NTF2 had not been implicated in binding to NPC proteins., Here we show that the D23A mutation in NTF2 does not result in gross, structural changes affecting other known NPC binding sites. Instead, the, D23 residue is located in an evolutionarily important region in the NTF2, domain containing superfamily, that in NTF2, is involved in binding to the, NPC.
<StructureSection load='1u5o' size='340' side='right'caption='[[1u5o]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1u5o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U5O FirstGlance]. <br>
1U5O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U5O OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5o OCA], [https://pdbe.org/1u5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u5o RCSB], [https://www.ebi.ac.uk/pdbsum/1u5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5o ProSAT]</span></td></tr>
==Reference==
</table>
Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2., Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS, J Mol Biol. 2004 Nov 19;344(2):303-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15522285 15522285]
== Function ==
[https://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u5o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u5o ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Bowman BR]]
[[Category: Bowman, B.R.]]
[[Category: Cushman I]]
[[Category: Cushman, I.]]
[[Category: Lichtarge O]]
[[Category: Lichtarge, O.]]
[[Category: Moore MS]]
[[Category: Moore, M.S.]]
[[Category: Quiocho FA]]
[[Category: Quiocho, F.A.]]
[[Category: Sowa ME]]
[[Category: Sowa, M.E.]]
[[Category: ntf2 mutant d23a]]
[[Category: nuclear transport protein]]
[[Category: transport]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:51:40 2007''

Latest revision as of 08:44, 14 February 2024

Structure of the D23A mutant of the nuclear transport carrier NTF2

1u5o, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1u5o&oldid=4055836"