1u9p: Difference between revisions

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New page: left|200px<br /><applet load="1u9p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u9p, resolution 1.9Å" /> '''Permuted single-chain...
 
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[[Image:1u9p.gif|left|200px]]<br /><applet load="1u9p" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1u9p, resolution 1.9&Aring;" />
'''Permuted single-chain Arc'''<br />


==Overview==
==Permuted single-chain Arc==
We designed a single-chain variant of the Arc repressor homodimer in which, the beta strands that contact operator DNA are connected by a hairpin turn, and the alpha helices that form the tetrahelical scaffold of the dimer are, attached by a short linker. The designed protein represents a noncyclic, permutation of secondary structural elements in another single-chain Arc, molecule (Arc-L1-Arc), in which the two subunits are fused by a single, linker. The permuted protein binds operator DNA with nanomolar affinity, refolds on the sub-millisecond time scale, and is as stable as Arc-L1-Arc., The crystal structure of the permuted protein reveals an essentially, wild-type fold, demonstrating that crucial folding information is not, encoded in the wild-type order of secondary structure. Noncyclic, rearrangement of secondary structure may allow grouping of critical, active-site residues in other proteins and could be a useful tool for, protein design and minimization.
<StructureSection load='1u9p' size='340' side='right'caption='[[1u9p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1u9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9P FirstGlance]. <br>
1U9P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U9P OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9p OCA], [https://pdbe.org/1u9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9p RCSB], [https://www.ebi.ac.uk/pdbsum/1u9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9p ProSAT]</span></td></tr>
==Reference==
</table>
Consolidating critical binding determinants by noncyclic rearrangement of protein secondary structure., Tabtiang RK, Cezairliyan BO, Grant RA, Cochrane JC, Sauer RT, Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2305-9. Epub 2005 Feb 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15689399 15689399]
== Function ==
[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u9p_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u9p ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Cezairliyan, B.O.]]
[[Category: Cezairliyan BO]]
[[Category: Cochrane, J.C.]]
[[Category: Cochrane JC]]
[[Category: Grant, R.A.]]
[[Category: Grant RA]]
[[Category: Sauer, R.T.]]
[[Category: Sauer RT]]
[[Category: Tabtiang, R.K.]]
[[Category: Tabtiang RK]]
[[Category: arc]]
[[Category: parc]]
 
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