2w1k: Difference between revisions
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==CRYSTAL STRUCTURE OF SORTASE C-3 (SRTC-3) from Streptococcus pneumoniae== | |||
<StructureSection load='2w1k' size='340' side='right'caption='[[2w1k]], [[Resolution|resolution]] 2.14Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2w1k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1K FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1k OCA], [https://pdbe.org/2w1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1k RCSB], [https://www.ebi.ac.uk/pdbsum/2w1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1k ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A7KT36_STREE A7KT36_STREE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Streptococcus pneumoniae is a piliated pathogen whose ability to circumvent vaccination and antibiotic treatment strategies is a cause of mortality worldwide. Pili play important roles in pneumococcal infection, but little is known about their biogenesis mechanism or the relationship between components of the pilus-forming machinery, which includes the fiber pilin (RrgB), two minor pilins (RrgA, RrgC), and three sortases (SrtC-1, SrtC-2, SrtC-3). Here we show that SrtC-1 is the main pilus-polymerizing transpeptidase, and electron microscopy analyses of RrgB fibers reconstituted in vitro reveal that they structurally mimic the pneumococcal pilus backbone. Crystal structures of both SrtC-1 and SrtC-3 reveal active sites whose access is controlled by flexible lids, unlike in non-pilus sortases, and suggest that substrate specificity is dictated by surface recognition coupled to lid opening. The distinct structural features of pilus-forming sortases suggest a common pilus biogenesis mechanism that could be exploited for the development of broad-spectrum antibacterials. | |||
Sortase-Mediated Pilus Fiber Biogenesis in Streptococcus pneumoniae.,Manzano C, Contreras-Martel C, El Mortaji L, Izore T, Fenel D, Vernet T, Schoehn G, Di Guilmi AM, Dessen A Structure. 2008 Dec 12;16(12):1838-48. PMID:19081060<ref>PMID:19081060</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2w1k" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus pneumoniae TIGR4]] | |||
[[Category: Contreras-Martel C]] | |||
[[Category: Dessen A]] | |||
[[Category: Di Guilmi AM]] | |||
[[Category: El Mortaji L]] | |||
[[Category: Fenel D]] | |||
[[Category: Izore T]] | |||
[[Category: Manzano C]] | |||
[[Category: Schoehn G]] | |||
[[Category: Vernet T]] | |||
Latest revision as of 15:40, 13 December 2023
CRYSTAL STRUCTURE OF SORTASE C-3 (SRTC-3) from Streptococcus pneumoniae
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