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New page: left|200px<br /><applet load="1v9e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1v9e, resolution 1.95Å" /> '''Crystal Structure An...
 
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[[Image:1v9e.gif|left|200px]]<br /><applet load="1v9e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1v9e, resolution 1.95&Aring;" />
'''Crystal Structure Analysis of Bovine Carbonic Anhydrase II'''<br />


==Overview==
==Crystal Structure Analysis of Bovine Carbonic Anhydrase II==
Carbonic anhydrase (CA) is a zinc-containing enzyme that catalyzes the, reversible hydration of CO2 to HCO3-. In eukaryotes, the enzyme plays a, role in various physiological functions, including interconversion between, CO2 and HCO3- in intermediary metabolism, facilitated diffusion of CO2, pH, homeostasis and ion transport. The structure of bovine carbonic anhydrase, II (BCA II) has been determined by molecular replacement and refined to, 1.95 A resolution by simulated-annealing and individual B-factor, refinement. The final R factor for the BCA II structure was 19.4%. BCA II, has a C-terminal knot structure similar to that observed in human CA II., It contains one zinc ion in the active site coordinated to three, histidines and one putative water molecule in a tetrahedral geometry. The, structure of BCA II reveals a probable alternative proton-wire pathway, that differs from that of HCA II.
<StructureSection load='1v9e' size='340' side='right'caption='[[1v9e]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1v9e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V9E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v9e OCA], [https://pdbe.org/1v9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v9e RCSB], [https://www.ebi.ac.uk/pdbsum/1v9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v9e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAH2_BOVIN CAH2_BOVIN] Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v9/1v9e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v9e ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carbonic anhydrase (CA) is a zinc-containing enzyme that catalyzes the reversible hydration of CO2 to HCO3-. In eukaryotes, the enzyme plays a role in various physiological functions, including interconversion between CO2 and HCO3- in intermediary metabolism, facilitated diffusion of CO2, pH homeostasis and ion transport. The structure of bovine carbonic anhydrase II (BCA II) has been determined by molecular replacement and refined to 1.95 A resolution by simulated-annealing and individual B-factor refinement. The final R factor for the BCA II structure was 19.4%. BCA II has a C-terminal knot structure similar to that observed in human CA II. It contains one zinc ion in the active site coordinated to three histidines and one putative water molecule in a tetrahedral geometry. The structure of BCA II reveals a probable alternative proton-wire pathway that differs from that of HCA II.


==About this Structure==
Structure of bovine carbonic anhydrase II at 1.95 A resolution.,Saito R, Sato T, Ikai A, Tanaka N Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):792-5. Epub 2004, Mar 23. PMID:15039588<ref>PMID:15039588</ref>
1V9E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V9E OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of bovine carbonic anhydrase II at 1.95 A resolution., Saito R, Sato T, Ikai A, Tanaka N, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):792-5. Epub 2004, Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15039588 15039588]
</div>
<div class="pdbe-citations 1v9e" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Carbonate dehydratase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Ikai A]]
[[Category: Ikai, A.]]
[[Category: Saito R]]
[[Category: Saito, R.]]
[[Category: Sato T]]
[[Category: Sato, T.]]
[[Category: Tanaka N]]
[[Category: Tanaka, N.]]
[[Category: ZN]]
[[Category: high-resolution]]
[[Category: twisted beta sheet]]
[[Category: zinc metalloenzyme]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 04:31:20 2007''

Latest revision as of 00:00, 28 December 2023

Crystal Structure Analysis of Bovine Carbonic Anhydrase II

1v9e, resolution 1.95Å

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