2w1t: Difference between revisions

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{{Seed}}
[[Image:2w1t.png|left|200px]]


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==Crystal Structure of B. subtilis SpoVT==
The line below this paragraph, containing "STRUCTURE_2w1t", creates the "Structure Box" on the page.
<StructureSection load='2w1t' size='340' side='right'caption='[[2w1t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2w1t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1T FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1t OCA], [https://pdbe.org/2w1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1t RCSB], [https://www.ebi.ac.uk/pdbsum/2w1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1t ProSAT]</span></td></tr>
{{STRUCTURE_2w1t|  PDB=2w1t  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPOVT_BACSU SPOVT_BACSU] Transcriptional factor that regulates positively or negatively the expression of a large number of forespore-specific sigma G-dependent genes. May provide a mechanism of feedback control that is important for forespore development (PubMed:8755877). SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores (PubMed:22522895).<ref>PMID:22522895</ref> <ref>PMID:8755877</ref>
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== Publication Abstract from PubMed ==
Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the crystal structure of the isolated C-terminal domain of SpoVT at 1.5 A by experimental phasing techniques and used this model to solve the structure of the full-length SpoVT at 2.6 A by molecular replacement. SpoVT is a tetramer that shows an overall significant distortion mediated by electrostatic interactions. Two monomers dimerize via the highly charged N-terminal domains to form swapped-hairpin beta-barrels. These asymmetric dimers further tetramerize through the formation of mixed helix bundles between their C-terminal domains, which themselves fold as GAF (cGMP-specific and cGMP-stimulated phosphodiesterases, Anabaena adenylate cyclases, and Escherichia coli FhlA) domains. The combination of a swapped-hairpin beta-barrel with a GAF domain represents a novel domain architecture in transcription factors. The occurrence of SpoVT homologs throughout Bacilli and Clostridia demonstrates the ancestral origin of this factor in sporulation.


===CRYSTAL STRUCTURE OF B. SUBTILIS SPOVT===
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis.,Asen I, Djuranovic S, Lupas AN, Zeth K J Mol Biol. 2008 Oct 30. PMID:18996130<ref>PMID:18996130</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 18996130 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_18996130}}
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</StructureSection>
==About this Structure==
2W1T is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1T OCA].
 
==Reference==
Crystal Structure of SpoVT, the Final Modulator of Gene Expression during Spore Development in Bacillus subtilis., Asen I, Djuranovic S, Lupas AN, Zeth K, J Mol Biol. 2008 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18996130 18996130]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Asen, I.]]
[[Category: Large Structures]]
[[Category: Djuranovic, S.]]
[[Category: Asen I]]
[[Category: Lupas, A N.]]
[[Category: Djuranovic S]]
[[Category: Zeth, K.]]
[[Category: Lupas AN]]
[[Category: Activator]]
[[Category: Zeth K]]
[[Category: Dna-binding]]
[[Category: Gaf domain regulated tetrameric spovt]]
[[Category: Repressor]]
[[Category: Sporulation]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 11:04:13 2008''

Latest revision as of 07:16, 9 August 2023

Crystal Structure of B. subtilis SpoVT

2w1t, resolution 2.60Å

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