2zpk: Difference between revisions

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{{Seed}}
[[Image:2zpk.jpg|left|200px]]


<!--
==Crystal structure of P20.1 Fab fragment in complex with its antigen peptide==
The line below this paragraph, containing "STRUCTURE_2zpk", creates the "Structure Box" on the page.
<StructureSection load='2zpk' size='340' side='right'caption='[[2zpk]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zpk]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZPK FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
{{STRUCTURE_2zpk|  PDB=2zpk  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zpk OCA], [https://pdbe.org/2zpk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zpk RCSB], [https://www.ebi.ac.uk/pdbsum/2zpk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zpk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PAR4_HUMAN PAR4_HUMAN] Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.<ref>PMID:10079109</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/2zpk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zpk ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Biologically important human proteins often require mammalian cell expression for structural studies, presenting technical and economical problems in the production/purification processes. We introduce a novel affinity peptide tagging system that uses a low affinity anti-peptide monoclonal antibody. Concatenation of the short recognition sequence enabled the successful engineering of an 18-residue affinity tag with ideal solution binding kinetics, providing a low-cost purification means when combined with nondenaturing elution by water-miscible organic solvents. Three-dimensional information provides a firm structural basis for the antibody-peptide interaction, opening opportunities for further improvements/modifications.


===Crystal structure of P20.1 Fab fragment in complex with its antigen peptide===
Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification.,Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J Protein Sci. 2008 Dec;17(12):2120-6. Epub 2008 Sep 11. PMID:18787202<ref>PMID:18787202</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zpk" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18787202}}, adds the Publication Abstract to the page
*[[Antibody 3D structures|Antibody 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18787202 is the PubMed ID number.
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
-->
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
{{ABSTRACT_PUBMED_18787202}}
== References ==
 
<references/>
==About this Structure==
__TOC__
2ZPK is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZPK OCA].
</StructureSection>
 
[[Category: Homo sapiens]]
==Reference==
[[Category: Large Structures]]
Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification., Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J, Protein Sci. 2008 Dec;17(12):2120-6. Epub 2008 Sep 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18787202 18787202]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Nogi, T.]]
[[Category: Nogi T]]
[[Category: Sanagawa, T.]]
[[Category: Sanagawa T]]
[[Category: Takagi, J.]]
[[Category: Takagi J]]
[[Category: Immune system]]
[[Category: Mouse igg fab fragment in complex with antigen peptide]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 24 11:27:39 2008''
Retrieved from "https://proteopedia.org/2zpk"