2zk7: Difference between revisions

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{{Seed}}
[[Image:2zk7.jpg|left|200px]]


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==Structure of a C-terminal deletion mutant of Thermoplasma acidophilum aldohexose dehydrogenase (AldT)==
The line below this paragraph, containing "STRUCTURE_2zk7", creates the "Structure Box" on the page.
<StructureSection load='2zk7' size='340' side='right'caption='[[2zk7]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zk7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZK7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.71&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zk7 OCA], [https://pdbe.org/2zk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zk7 RCSB], [https://www.ebi.ac.uk/pdbsum/2zk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zk7 ProSAT]</span></td></tr>
{{STRUCTURE_2zk7|  PDB=2zk7  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HK51_THEAC Q9HK51_THEAC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zk/2zk7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zk7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The D-aldohexose dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum (AldT) is a homotetrameric enzyme that catalyzes the oxidation of several D-aldohexoses, especially D-mannose. AldT comprises a unique C-terminal tail motif (residues 247-255) that shuts the active-site pocket of the neighboring subunit. The functional role of the C-terminal tail of AldT has been investigated using mutational and crystallographic analyses. A total of four C-terminal deletion mutants (Delta254, Delta253, Delta252, and Delta249) and two site-specific mutants (Y86G and P254G) were expressed by Escherichia coli and purified. Enzymatic characterization of these mutants revealed that the C-terminal tail is a requisite and that the interaction between Tyr86 and Pro254 is critical for enzyme activity. The crystal structure of the Delta249 mutant was also determined. The structure showed that the active-site loops undergo a significant conformational change, which leads to the structural deformation of the substrate-binding pocket. Proteins 2009. (c) 2008 Wiley-Liss, Inc.


===Structure of a C-terminal deletion mutant of Thermoplasma acidophilum aldohexose dehydrogenase (AldT)===
C-terminal tail derived from the neighboring subunit is critical for the activity of Thermoplasma acidophilumD-aldohexose dehydrogenase.,Nishioka T, Yasutake Y, Nishiya Y, Tamura N, Tamura T Proteins. 2008 Oct 21. PMID:19089950<ref>PMID:19089950</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_19089950}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2zk7" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 19089950 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_19089950}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2ZK7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZK7 OCA].
 
==Reference==
C-terminal tail derived from the neighboring subunit is critical for the activity of Thermoplasma acidophilumD-aldohexose dehydrogenase., Nishioka T, Yasutake Y, Nishiya Y, Tamura N, Tamura T, Proteins. 2008 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19089950 19089950]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
[[Category: Nishioka, T.]]
[[Category: Nishioka T]]
[[Category: Nishiya, Y.]]
[[Category: Nishiya Y]]
[[Category: Tamura, N.]]
[[Category: Tamura N]]
[[Category: Tamura, T.]]
[[Category: Tamura T]]
[[Category: Yasutake, Y.]]
[[Category: Yasutake Y]]
[[Category: Oxidoreductase]]
[[Category: Rossmann fold]]
 
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Retrieved from "https://proteopedia.org/2zk7"