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New page: left|200px<br /><applet load="1wpu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wpu, resolution 1.48Å" /> '''Crystal Structure of...
 
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[[Image:1wpu.gif|left|200px]]<br /><applet load="1wpu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wpu, resolution 1.48&Aring;" />
'''Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA'''<br />


==Overview==
==Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA==
HutP is an L-histidine-activated RNA binding protein that regulates the, expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on the hut mRNA. The crystal, structure of HutP complexed with an L-histidine analog showed a novel, fold; there are four antiparallel beta strands in the central region of, each monomer, with two alpha helices each on the front and back. Two HutP, monomers form a dimer, and three dimers are arranged in crystallographic, 3-fold symmetry to form a hexamer. A histidine analog was located in, between the two monomers of HutP, with the imidazole group of L-histidine, hydrogen bonded to Glu81. An activation mechanism is proposed based on the, identification of key residues of HutP. The HutP binding region in hut, mRNA was defined: it consists of three UAG trinucleotide motifs separated, by four spacer nucleotides. Residues of HutP potentially important for RNA, binding were identified.
<StructureSection load='1wpu' size='340' side='right'caption='[[1wpu]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1wpu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WPU FirstGlance]. <br>
1WPU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with MG and HIS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WPU OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wpu OCA], [https://pdbe.org/1wpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wpu RCSB], [https://www.ebi.ac.uk/pdbsum/1wpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wpu ProSAT]</span></td></tr>
Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242603 15242603]
</table>
== Function ==
[https://www.uniprot.org/uniprot/HUTP_BACSU HUTP_BACSU] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization.[HAMAP-Rule:MF_00779]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/1wpu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wpu ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kumar, P.K.R.]]
[[Category: Kumar PKR]]
[[Category: Kumarevel, T.S.]]
[[Category: Kumarevel TS]]
[[Category: Mizuno, H.]]
[[Category: Mizuno H]]
[[Category: HIS]]
[[Category: MG]]
[[Category: antitermination]]
[[Category: hutp]]
[[Category: hutp-rna complex]]
[[Category: rna binding]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:35:36 2007''

Latest revision as of 07:57, 25 October 2023

Crystal Structure of the HutP antitermination complex bound to a single stranded region of hut mRNA

1wpu, resolution 1.48Å

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