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New page: left|200px<br /><applet load="1ws7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ws7, resolution 1.90Å" /> '''Crystal Structure of...
 
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[[Image:1ws7.jpg|left|200px]]<br /><applet load="1ws7" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ws7, resolution 1.90&Aring;" />
'''Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)'''<br />


==Overview==
==Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)==
Mavicyanin, a glycosylated protein isolated from Cucurbita pepo medullosa, (zucchini), is a member of the phytocyanin subfamily containing one, polypeptide chain of 109 amino residues and an unusual type-I Cu site in, which the copper ligands are His45, Cys86, His91, and Gln96. The crystal, structures of oxidized and reduced mavicyanin were determined at 1.6 and, 1.9 A resolution, respectively. Mavicyanin has a core structure of seven, polypeptide beta-strands arranged as a beta-sandwich organized into two, beta-sheets, and the structure considerably resembles that of stellacyanin, from cucumber (CST) or cucumber basic protein (CBP). A flexible region was, not observed on superimpositioning of the oxidized and reduced mavicyanin, structures. However, the Cu(II)-epsilon-O-Gln96 bond length was extended, by 0.47 A, and the Thr15 residue was rotated by 60.0 degrees and, O-gamma1-Thr15 moved from a distance of 4.78 to 2.58 A from the ligand, Gln96 forming a new hydrogen bond between O-gamma1-Thr15 and, epsilon-O-Gln96 upon reduction. The reorganization of copper coordination, geometry of mavicyanin upon reduction arouses reduction potential, decreased above pH 8 [Battistuzzi et al. (2001) J. Inorg. Biochem. 83, 223-227]. The rotation of Thr15 and the hydrogen bonding with the ligand, Gln96 may constitute structural evidence of the decrease in the reduction, potential at high pH.
<StructureSection load='1ws7' size='340' side='right'caption='[[1ws7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ws7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucurbita_pepo Cucurbita pepo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WS7 FirstGlance]. <br>
1WS7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cucurbita_pepo Cucurbita pepo] with CU1 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WS7 OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ws7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ws7 OCA], [https://pdbe.org/1ws7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ws7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ws7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ws7 ProSAT]</span></td></tr>
Structural reorganization of the copper binding site involving Thr15 of mavicyanin from Cucurbita pepo medullosa (zucchini) upon reduction., Xie Y, Inoue T, Miyamoto Y, Matsumura H, Kataoka K, Yamaguchi K, Nojini M, Suzuki S, Kai Y, J Biochem (Tokyo). 2005 Apr;137(4):455-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15858169 15858169]
</table>
== Function ==
[https://www.uniprot.org/uniprot/MAVI_CUCPE MAVI_CUCPE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1ws7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ws7 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Cucurbita pepo]]
[[Category: Cucurbita pepo]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Inoue, T.]]
[[Category: Inoue T]]
[[Category: Kai, Y.]]
[[Category: Kai Y]]
[[Category: Kataoka, K.]]
[[Category: Kataoka K]]
[[Category: Matsumura, H.]]
[[Category: Matsumura H]]
[[Category: Miyamoto, Y.]]
[[Category: Miyamoto Y]]
[[Category: Nojini, M.]]
[[Category: Nojini M]]
[[Category: Suzuki, S.]]
[[Category: Suzuki S]]
[[Category: Xie, Y.]]
[[Category: Xie Y]]
[[Category: Yamaguchi, K.]]
[[Category: Yamaguchi K]]
[[Category: CU1]]
[[Category: cupredoxin]]
[[Category: mavicyanin]]
[[Category: phytocyanin]]
[[Category: reduced form]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:38:23 2007''

Latest revision as of 06:18, 3 April 2024

Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)

1ws7, resolution 1.90Å

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