2zqn: Difference between revisions

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{{Seed}}
[[Image:2zqn.png|left|200px]]


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==Crystal structure of the earthworm R-type lectin C-half in complex with Lactose==
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<StructureSection load='2zqn' size='340' side='right'caption='[[2zqn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zqn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2d12 2d12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZQN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
{{STRUCTURE_2zqn|  PDB=2zqn  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zqn OCA], [https://pdbe.org/2zqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zqn RCSB], [https://www.ebi.ac.uk/pdbsum/2zqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zqn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O96048_LUMTE O96048_LUMTE]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
R-type lectins are one of the most prominent types of lectin; they exist ubiquitously in nature and mainly bind to the galactose unit of sugar chains. The galactose-binding lectin EW29 from the earthworm Lumbricus terrestris belongs to the R-type lectin family as represented by the plant lectin ricin. It shows haemagglutination activity and is composed of a single peptide chain that includes two homologous domains: N-terminal and C-terminal domains. A truncated mutant of EW29 comprising the C-terminal domain (rC-half) has haemagglutination activity by itself. In order to clarify how rC-half recognizes ligands and shows haemagglutination activity, X-ray crystal structures of rC-half in complex with D-lactose and N-acetyl-D-galactosamine have been determined. The structure of rC-half is similar to that of the ricin B chain and consists of a beta-trefoil fold; the fold is further divided into three similar subdomains referred to as subdomains alpha, beta and gamma, which are gathered around the pseudo-threefold axis. The structures of sugar complexes demonstrated that subdomains alpha and gamma of rC-half bind terminal galactosyl and N-acetylgalactosaminyl glycans. The sugar-binding properties are common to both ligands in both subdomains and are quite similar to those of ricin B chain-lactose complexes. These results indicate that the C-terminal domain of EW29 uses these two galactose-binding sites for its function as a single-domain-type haemagglutinin.


===Crystal structure of the earthworm R-type lectin C-half in complex with Lactose===
Sugar-complex structures of the C-half domain of the galactose-binding lectin EW29 from the earthworm Lumbricus terrestris.,Suzuki R, Kuno A, Hasegawa T, Hirabayashi J, Kasai KI, Momma M, Fujimoto Z Acta Crystallogr D Biol Crystallogr. 2009 Jan;65(Pt 1):49-57. Epub 2008, Dec 18. PMID:19153466<ref>PMID:19153466</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zqn" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_15388944}}, adds the Publication Abstract to the page
*[[Galactose-binding lectin|Galactose-binding lectin]]
(as it appears on PubMed at http://www.pubmed.gov), where 15388944 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_15388944}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2ZQN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQN OCA].
 
==Reference==
Crystallization and preliminary X-ray crystallographic studies of the C-terminal domain of galactose-binding lectin EW29 from the earthworm Lumbricus terrestris., Suzuki R, Fujimoto Z, Kuno A, Hirabayashi J, Kasai K, Hasegawa T, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1895-6. Epub 2004, Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15388944 15388944]
 
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683]
 
Novel galactose-binding proteins in Annelida. Characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris., Hirabayashi J, Dutta SK, Kasai K, J Biol Chem. 1998 Jun 5;273(23):14450-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9603958 9603958]
[[Category: Lumbricus terrestris]]
[[Category: Lumbricus terrestris]]
[[Category: Single protein]]
[[Category: Fujimoto Z]]
[[Category: Fujimoto, Z.]]
[[Category: Hasegawa T]]
[[Category: Hasegawa, T.]]
[[Category: Hirabayashi J]]
[[Category: Hirabayashi, J.]]
[[Category: Kasai K]]
[[Category: Kasai, K.]]
[[Category: Kuno A]]
[[Category: Kuno, A.]]
[[Category: Momma M]]
[[Category: Momma, M.]]
[[Category: Suzuki R]]
[[Category: Suzuki, R.]]
[[Category: Beta-trefoil fold]]
[[Category: Earthworm lumbricus terrestri]]
[[Category: Hemagglutinin]]
[[Category: Lectin]]
[[Category: R-type lectin]]
[[Category: Sugar binding protein]]
[[Category: Sugar complex]]
 
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Retrieved from "https://proteopedia.org/2zqn"