1wvl: Difference between revisions
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New page: left|200px<br /><applet load="1wvl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wvl, resolution 2.6Å" /> '''Crystal Structure of ... |
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== | ==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer== | ||
The protein Sac7d belongs to a class of small chromosomal proteins from | <StructureSection load='1wvl' size='340' side='right'caption='[[1wvl]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WVL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [https://pdbe.org/1wvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [https://www.ebi.ac.uk/pdbsum/1wvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvl ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties. | |||
Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates.,Wu SW, Ko TP, Chou CC, Wang AH Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219<ref>PMID:16028219</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1wvl" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Gcn4 3D Structures|Gcn4 3D Structures]] | |||
*[[Gnc4 3D Structures|Gnc4 3D Structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sulfolobus acidocaldarius]] | [[Category: Sulfolobus acidocaldarius]] | ||
[[Category: Wang | [[Category: Wang AH]] | ||
[[Category: Wu | [[Category: Wu SW]] | ||
Latest revision as of 11:25, 2 August 2023
Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer
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