1xfn: Difference between revisions
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New page: left|200px<br /><applet load="1xfn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xfn" /> '''NMR structure of the ground state of the pho... |
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== | ==NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part== | ||
The N-terminally truncated variant of photoactive yellow protein | <StructureSection load='1xfn' size='340' side='right'caption='[[1xfn]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1xfn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XFN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xfn OCA], [https://pdbe.org/1xfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xfn RCSB], [https://www.ebi.ac.uk/pdbsum/1xfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xfn ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xf/1xfn_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xfn ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated. | |||
The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein.,Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA Structure. 2005 Jul;13(7):953-62. PMID:16004868<ref>PMID:16004868</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1xfn" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Halorhodospira halophila]] | [[Category: Halorhodospira halophila]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Bernard | [[Category: Bernard C]] | ||
[[Category: Boelens | [[Category: Boelens R]] | ||
[[Category: Derix | [[Category: Derix NM]] | ||
[[Category: Hellingwerf | [[Category: Hellingwerf KJ]] | ||
[[Category: Houben K]] | |||
[[Category: Houben | [[Category: Kaptein R]] | ||
[[Category: Kaptein | [[Category: Marks D]] | ||
[[Category: Marks | [[Category: Van Nuland NA]] | ||
[[Category: Nuland | [[Category: Van der Horst MA]] | ||
[[Category: | |||
Latest revision as of 14:07, 9 May 2024
NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part
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