1p77: Difference between revisions

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{{Seed}}
[[Image:1p77.png|left|200px]]


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==CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE==
The line below this paragraph, containing "STRUCTURE_1p77", creates the "Structure Box" on the page.
<StructureSection load='1p77' size='340' side='right'caption='[[1p77]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1p77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P77 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ATR:2-MONOPHOSPHOADENOSINE-5-DIPHOSPHATE'>ATR</scene></td></tr>
{{STRUCTURE_1p77|  PDB=1p77  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p77 OCA], [https://pdbe.org/1p77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p77 RCSB], [https://www.ebi.ac.uk/pdbsum/1p77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p77 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROE_HAEIN AROE_HAEIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p77_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p77 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Shikimate dehydrogenase catalyzes the NADPH-dependent reversible reduction of 3-dehydroshikimate to shikimate. We report the first X-ray structure of shikimate dehydrogenase from Haemophilus influenzae to 2.4-A resolution and its complex with NADPH to 1.95-A resolution. The molecule contains two domains, a catalytic domain with a novel open twisted alpha/beta motif and an NADPH binding domain with a typical Rossmann fold. The enzyme contains a unique glycine-rich P-loop with a conserved sequence motif, GAGGXX, that results in NADPH adopting a nonstandard binding mode with the nicotinamide and ribose moieties disordered in the binary complex. A deep pocket with a narrow entrance between the two domains, containing strictly conserved residues primarily contributed by the catalytic domain, is identified as a potential 3-dehydroshikimate binding pocket. The flexibility of the nicotinamide mononucleotide portion of NADPH may be necessary for the substrate 3-dehydroshikimate to enter the pocket and for the release of the product shikimate.


===CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE===
The crystal structure of shikimate dehydrogenase (AroE) reveals a unique NADPH binding mode.,Ye S, Von Delft F, Brooun A, Knuth MW, Swanson RV, McRee DE J Bacteriol. 2003 Jul;185(14):4144-51. PMID:12837789<ref>PMID:12837789</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1p77" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_12837789}}, adds the Publication Abstract to the page
*[[Shikimate dehydrogenase 3D structures|Shikimate dehydrogenase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 12837789 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_12837789}}
__TOC__
 
</StructureSection>
==About this Structure==
1P77 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P77 OCA].
 
==Reference==
<ref group="xtra">PMID:12837789</ref><references group="xtra"/>
[[Category: Haemophilus influenzae]]
[[Category: Shikimate dehydrogenase]]
[[Category: Brooun, A.]]
[[Category: Delft, F von.]]
[[Category: Knuth, M W.]]
[[Category: McRee, D E.]]
[[Category: Swanson, R V.]]
[[Category: Ye, S.]]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Nadph]]
[[Category: Large Structures]]
[[Category: Shikimate dehydrogenase]]
[[Category: Brooun A]]
 
[[Category: Knuth MW]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:05:17 2009''
[[Category: McRee DE]]
[[Category: Swanson RV]]
[[Category: Ye S]]
[[Category: Von Delft F]]
Retrieved from "https://proteopedia.org/1p77"