2axe: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(11 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:2axe.png|left|200px]]


<!--
==IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS==
The line below this paragraph, containing "STRUCTURE_2axe", creates the "Structure Box" on the page.
<StructureSection load='2axe' size='340' side='right'caption='[[2axe]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2axe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Talaromyces_purpureogenus Talaromyces purpureogenus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AXE FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TYI:3,5-DIIODOTYROSINE'>TYI</scene></td></tr>
{{STRUCTURE_2axe|  PDB=2axe  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2axe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2axe OCA], [https://pdbe.org/2axe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2axe RCSB], [https://www.ebi.ac.uk/pdbsum/2axe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2axe ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AXE2_TALPU AXE2_TALPU] Degrades acetylated xylans by cleaving acetyl side groups from the hetero-xylan backbone.<ref>PMID:8756392</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/2axe_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2axe ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymatic and non-enzymatic iodination of the amino acid tyrosine is a well known phenomenon. The iodination technique has been widely used for labeling proteins. Using high-resolution X-ray crystallographic techniques, the chemical and three-dimensional structures of iodotyrosines formed by non-enzymatic incorporation of I atoms into tyrosine residues of a crystalline protein are described. Acetylxylan esterase (AXE II; 207 amino-acid residues) from Penicillium purpurogenum has substrate specificities towards acetate esters of D-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. The crystals of the enzyme are highly ordered, tightly packed and diffract to better than sub-angstrom resolution at 85 K. The iodination technique has been utilized to prepare an isomorphous derivative of the AXE II crystal. The structure of the enzyme determined at 1.10 A resolution exclusively by normal and anomalous scattering from I atoms, along with the structure of the iodinated complex at 1.80 A resolution, demonstrate the formation of covalent bonds between I atoms and C atoms at ortho positions to the hydroxyl groups of two tyrosyl moieties, yielding iodotyrosines.


===IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS===
Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase.,Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:10089308<ref>PMID:10089308</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2axe" style="background-color:#fffaf0;"></div>


<!--
==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_10089308}}, adds the Publication Abstract to the page
*[[Acetylxylan esterase 3D structures|Acetylxylan esterase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 10089308 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_10089308}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2AXE is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA].
[[Category: Talaromyces purpureogenus]]
 
[[Category: Erman M]]
==Reference==
[[Category: Eyzaguirre J]]
<ref group="xtra">PMID:10089308</ref><references group="xtra"/>
[[Category: Ghosh D]]
[[Category: Acetylesterase]]
[[Category: Jornvall H]]
[[Category: Penicillium purpurogenum]]
[[Category: Lala P]]
[[Category: Erman, M.]]
[[Category: Li N]]
[[Category: Eyzaguirre, J.]]
[[Category: Pangborn W]]
[[Category: Ghosh, D.]]
[[Category: Sawicki MW]]
[[Category: Jornvall, H.]]
[[Category: Thiel DJ]]
[[Category: Lala, P.]]
[[Category: Weeks DR]]
[[Category: Li, N.]]
[[Category: Pangborn, W.]]
[[Category: Sawicki, M W.]]
[[Category: Thiel, D J.]]
[[Category: Weeks, D R.]]
[[Category: Esterase]]
[[Category: Hydrolase]]
[[Category: Iodotyrosine]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:18:30 2009''
Retrieved from "https://proteopedia.org/2axe"