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New page: left|200px<br /><applet load="1xkz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xkz, resolution 1.75Å" /> '''Crystal structure of...
 
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[[Image:1xkz.gif|left|200px]]<br /><applet load="1xkz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xkz, resolution 1.75&Aring;" />
'''Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus'''<br />


==Overview==
==Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus==
Methicillin-resistant strains of Staphylococcus aureus (MRSA) are the major cause of infections worldwide. Transcription of the -lactamase and PBP2a resistance genes is mediated by two closely related signal-transducing integral membrane proteins, BlaR1 and MecR1, upon binding of the -lactam inducer to the sensor domain. Herein we report the crystal structure at 1.75 &Aring; resolution of the sensor domain of BlaR1 in complex with a cephalosporin antibiotic. Activation of the signal transducer involves acylation of serine 389 by the -lactam antibiotic, a process promoted by the N-carboxylated side chain of Lys392. We present evidence that, on acylation, the lysine side chain experiences a spontaneous decarboxylation that entraps the sensor in its activated state. Kinetic determinations and quantum mechanical/molecular mechanical calculations and the interaction networks in the crystal structure shed light on how this unprecedented process for activation of a receptor may be achieved and provide insights into the mechanistic features that differentiate the signal-transducing receptor from the structurally related class D -lactamases, enzymes of antibiotic resistance.
<StructureSection load='1xkz' size='340' side='right'caption='[[1xkz]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1xkz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XKZ FirstGlance]. <br>
1XKZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with SO4, CAZ and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XKZ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAZ:ACYLATED+CEFTAZIDIME'>CAZ</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xkz OCA], [https://pdbe.org/1xkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xkz RCSB], [https://www.ebi.ac.uk/pdbsum/1xkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xkz ProSAT]</span></td></tr>
X-ray crystal structure of the acylated beta-lactam sensor domain of BlaR1 from Staphylococcus aureus and the mechanism of receptor activation for signal transduction., Birck C, Cha JY, Cross J, Schulze-Briese C, Meroueh SO, Schlegel HB, Mobashery S, Samama JP, J Am Chem Soc. 2004 Nov 3;126(43):13945-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15506754 15506754]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/BLAR_STAAU BLAR_STAAU] BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xk/1xkz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xkz ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Birck, C.]]
[[Category: Birck C]]
[[Category: Cha, J.Y.]]
[[Category: Cha JY]]
[[Category: Cross, J.]]
[[Category: Cross J]]
[[Category: Meroueh, S.O.]]
[[Category: Meroueh SO]]
[[Category: Mobashery, S.]]
[[Category: Mobashery S]]
[[Category: Samama, J.P.]]
[[Category: Samama J-P]]
[[Category: Schlegel, H.B.]]
[[Category: Schlegel HB]]
[[Category: Schulze-Briese, C.]]
[[Category: Schulze-Briese C]]
[[Category: CAZ]]
[[Category: EPE]]
[[Category: SO4]]
[[Category: beta-lactam receptor]]
[[Category: signal transduction]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:08:12 2007''

Latest revision as of 06:19, 3 April 2024

Crystal structure of the acylated beta-lactam sensor domain of Blar1 from S. aureus

1xkz, resolution 1.75Å

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