1xmt: Difference between revisions

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New page: left|200px<br /><applet load="1xmt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xmt, resolution 1.150Å" /> '''X-ray structure of ...
 
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[[Image:1xmt.gif|left|200px]]<br /><applet load="1xmt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xmt, resolution 1.150&Aring;" />
'''X-ray structure of gene product from arabidopsis thaliana at1g77540'''<br />


==Overview==
==X-ray structure of gene product from arabidopsis thaliana at1g77540==
We describe X-ray crystal and NMR solution structures of the protein coded, for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal, structure was determined to 1.15 A with an R factor of 14.9% (Rfree =, 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide, derivatized crystals. The ensemble of NMR conformers was determined with, protein samples labeled with 15N and 13C + 15N. The X-ray structure and, NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93., At1g77540 was found to adopt a fold similar to that of GCN5-related, N-acetyltransferases. Enzymatic activity assays established that At1g77540, possesses weak acetyltransferase activity against histones H3 and H4., Chemical shift perturbations observed in 15N-HSQC spectra upon the, addition of CoA indicated that the cofactor binds and identified its, binding site. The molecular details of this interaction were further, elucidated by solving the X-ray structure of the At1g77540-CoA complex., This work establishes that the domain family COG2388 represents a novel, class of acetyltransferase and provides insight into possible mechanistic, roles of the conserved Cys76 and His41 residues of this family.
<StructureSection load='1xmt' size='340' side='right'caption='[[1xmt]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1xmt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XMT FirstGlance]. <br>
1XMT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with BR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xmt OCA], [https://pdbe.org/1xmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xmt RCSB], [https://www.ebi.ac.uk/pdbsum/1xmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xmt ProSAT]</span></td></tr>
Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17128971 17128971]
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y1754_ARATH Y1754_ARATH] Possesses in vitro histone acetyltransferase activity with histones H3 and H4.<ref>PMID:17128971</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xm/1xmt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xmt ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Allard, S.T.M.]]
[[Category: Allard STM]]
[[Category: Bingman, C.A.]]
[[Category: Bingman CA]]
[[Category: Bitto, E.]]
[[Category: Bitto E]]
[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]
[[Category: Phillips Jr GN]]
[[Category: Jr., G.N.Phillips.]]
[[Category: Smith DW]]
[[Category: Smith, D.W.]]
[[Category: Wesenberg GE]]
[[Category: Wesenberg, G.E.]]
[[Category: BR]]
[[Category: at1g77540]]
[[Category: center for eukaryotic structural genomics]]
[[Category: cesg]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: putative acetyltransferase]]
[[Category: structural genomics]]
 
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Retrieved from "https://proteopedia.org/1xmt"