2jic: Difference between revisions

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{{Seed}}
[[Image:2jic.png|left|200px]]


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==High resolution structure of xylanase-II from one micron beam experiment==
The line below this paragraph, containing "STRUCTURE_2jic", creates the "Structure Box" on the page.
<StructureSection load='2jic' size='340' side='right'caption='[[2jic]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2jic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_longibrachiatum Trichoderma longibrachiatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JIC FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jic OCA], [https://pdbe.org/2jic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jic RCSB], [https://www.ebi.ac.uk/pdbsum/2jic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jic ProSAT]</span></td></tr>
{{STRUCTURE_2jic|  PDB=2jic  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/F8W669_TRILO F8W669_TRILO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/2jic_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jic ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
For the first time, protein microcrystallography has been performed with a focused synchrotron-radiation beam of 1 microm using a goniometer with a sub-micrometre sphere of confusion. The crystal structure of xylanase II has been determined with a flux density of about 3 x 10(10) photons s(-1) microm(-2) at the sample. Two sets of diffraction images collected from different sized crystals were shown to comprise data of good quality, which allowed a 1.5 A resolution xylanase II structure to be obtained. The main conclusion of this experiment is that a high-resolution diffraction pattern can be obtained from 20 microm(3) crystal volume, corresponding to about 2 x 10(8) unit cells. Despite the high irradiation dose in this case, it was possible to obtain an excellent high-resolution map and it could be concluded from the individual atomic B-factor patterns that there was no evidence of significant radiation damage. The photoelectron escape from a narrow diffraction channel is a possible reason for reduced radiation damage as indicated by Monte Carlo simulations. These results open many new opportunities in scanning protein microcrystallography and make random data collection from microcrystals a real possibility, therefore enabling structures to be solved from much smaller crystals than previously anticipated as long as the crystallites are well ordered.


===HIGH RESOLUTION STRUCTURE OF XYLANASE-II FROM ONE MICRON BEAM EXPERIMENT===
Protein crystallography with a micrometre-sized synchrotron-radiation beam.,Moukhametzianov R, Burghammer M, Edwards PC, Petitdemange S, Popov D, Fransen M, McMullan G, Schertler GF, Riekel C Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):158-66. Epub 2008, Jan 16. PMID:18219115<ref>PMID:18219115</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_18219115}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2jic" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 18219115 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18219115}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2JIC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Trichoderma_longibrachiatum Trichoderma longibrachiatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JIC OCA].
 
==Reference==
<ref group="xtra">PMID:18219115</ref><references group="xtra"/>
[[Category: Endo-1,4-beta-xylanase]]
[[Category: Trichoderma longibrachiatum]]
[[Category: Trichoderma longibrachiatum]]
[[Category: Burghammer, M.]]
[[Category: Burghammer M]]
[[Category: Edwards, P C.]]
[[Category: Edwards PC]]
[[Category: Fransen, M.]]
[[Category: Fransen M]]
[[Category: Moukhametzianov, R.]]
[[Category: Moukhametzianov R]]
[[Category: Petitdemange, S.]]
[[Category: Petitdemange S]]
[[Category: Popov, D.]]
[[Category: Popov D]]
[[Category: Riekel, C.]]
[[Category: Riekel C]]
[[Category: Schertler, G F.]]
[[Category: Schertler GF]]
[[Category: Endonuclease]]
[[Category: Fungi]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Microbeam]]
[[Category: Xylan]]
[[Category: Xylan degradation]]
 
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Retrieved from "https://proteopedia.org/2jic"