1xod: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="1xod" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xod, resolution 1.15Å" /> '''Crystal structure of...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1xod.gif|left|200px]]<br /><applet load="1xod" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xod, resolution 1.15&Aring;" />
'''Crystal structure of X. tropicalis Spred1 EVH-1 domain'''<br />


==Overview==
==Crystal structure of X. tropicalis Spred1 EVH-1 domain==
The recently described Spred protein family has been implicated in the, modulation of receptor tyrosine kinase signalling. We report the crystal, structure of the Enabled/vasodilator-stimulated phosphoprotein homology-1, (EVH1) domain from Xenopus tropicalis Spred1, solved to 1.15 A resolution., This structure confirms that the Spred EVH1 adopts the pleckstrin-homology, fold, with a similar secondary structure to Enabled. A translation of one, of the peptide-binding groove beta-strands narrows this groove, whilst one, end of the groove shows structural flexibility. We propose that Spred1, will bind peptides that are less proline-rich than other EVH1 domains, with conformational changes indicating an induced fit.
<StructureSection load='1xod' size='340' side='right'caption='[[1xod]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xod]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_tropicalis Xenopus tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XOD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xod OCA], [https://pdbe.org/1xod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xod RCSB], [https://www.ebi.ac.uk/pdbsum/1xod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xod ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPRE1_XENTR SPRE1_XENTR] Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xo/1xod_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xod ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recently described Spred protein family has been implicated in the modulation of receptor tyrosine kinase signalling. We report the crystal structure of the Enabled/vasodilator-stimulated phosphoprotein homology-1 (EVH1) domain from Xenopus tropicalis Spred1, solved to 1.15 A resolution. This structure confirms that the Spred EVH1 adopts the pleckstrin-homology fold, with a similar secondary structure to Enabled. A translation of one of the peptide-binding groove beta-strands narrows this groove, whilst one end of the groove shows structural flexibility. We propose that Spred1 will bind peptides that are less proline-rich than other EVH1 domains, with conformational changes indicating an induced fit.


==About this Structure==
1.15 A crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family.,Harmer NJ, Sivak JM, Amaya E, Blundell TL FEBS Lett. 2005 Feb 14;579(5):1161-6. PMID:15710406<ref>PMID:15710406</ref>
1XOD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_tropicalis Xenopus tropicalis] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XOD OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1.15 A crystal structure of the X. tropicalis Spred1 EVH1 domain suggests a fourth distinct peptide-binding mechanism within the EVH1 family., Harmer NJ, Sivak JM, Amaya E, Blundell TL, FEBS Lett. 2005 Feb 14;579(5):1161-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15710406 15710406]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1xod" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xenopus tropicalis]]
[[Category: Xenopus tropicalis]]
[[Category: Amaya, E.]]
[[Category: Amaya E]]
[[Category: Blundell, T.L.]]
[[Category: Blundell TL]]
[[Category: Harmer, N.J.]]
[[Category: Harmer NJ]]
[[Category: Sivak, J.M.]]
[[Category: Sivak JM]]
[[Category: GOL]]
[[Category: evh1]]
[[Category: peptide-binding]]
[[Category: spred]]
[[Category: sprouty]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:14:10 2007''
Retrieved from "https://proteopedia.org/1xod"